Plant RNA Isolation Aid

Catalog number: AM9690

Ambion™  Related applications: RNA Extraction

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Description

Recommended for use with the RNAqueous® Kit for purification of total RNA from plant tissues. One bottle of 10 mL Ambion® reagent is provided.

• Removes common plant contaminants such as polyphenolics and polysaccharides
• No phenol extraction, alcohol precipitation, or proteinase digestion steps required when used with the RNAqueous® kit

Ambion's Plant RNA Isolation Aid contains polyvinylpyrrolidone (PVP), a high molecular weight polymer that binds to contaminants such as polyphenolics and polysaccharides that are commonly present in plant tissues. Glass fiber filter–based methods of isolation, such as the RNAqueous® Kit, are especially suited for plant RNA isolation because it does not include any alcohol precipitation steps; many common contaminants found in plant tissue are known to co-precipitate with nucleic acid during alcohol precipitation. When used with the RNAqueous® Kit, the procedure consists of disrupting the plant tissue in a guanidinium-based lysis solution to which the Plant RNA Isolation Aid has been added. A brief centrifugation then removes the contaminants. The supernatant is passed through a glass fiber filter which binds RNA. The filter is then washed and the RNA is eluted in a small volume (50 µL or more) of elution solution.

Accessory Product:
The Plant RNA Isolation Aid is designed for use with the RNAqueous® Kit (SKU #AM1912) for RNA purification from plant tissues.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Plant Samples
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Product Size: 10 mL
Final Product: RNA Supernatant (for use with RNAqueous® kit)
Compatible Products: RNAqueous® Kit
Isolation Technology: Polyvinylpyrrolidone (PVP) Reagent
Shipping Condition: Room Temperature

Contents & storage

Store Plant RNA Isolation Aid at room temperature or at 4°C.

Documents

Manuals & protocols

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