TRI Reagent™ Solution
TRI Reagent™ Solution
Invitrogen™

TRI Reagent™ Solution

Tri Reagent™ solution is a complete, ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA,Read more
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Catalog NumberQuantity
AM9738100 mL
Catalog number AM9738
Price (USD)
251.00
Each
Add to cart
Quantity:
100 mL
Price (USD)
251.00
Each
Add to cart
Tri Reagent™ solution is a complete, ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. Key features:

Simple—a single reagent for isolation of DNA-free RNA, RNA-free DNA, and protein
Productive—obtain higher yields than with traditional guanidine thiocyanate or cesium chloride methods
Versatile—works with many sample sources, including human, plant, yeast, bacterial and viral samples
Flexible—easily scalable depending on starting material and desired yield

Using TRI Reagent™ solution
TRI Reagent™ starting material can be either fresh or frozen tissue, or cell samples of almost any size—the protocol is easily scalable. This RNA isolation method is well-recognized and referenced in numerous publications, a testament to its reliability.

How it works
TRI Reagent™ combines phenol and guanidine thiocyanate in a monophase solution to facilitate the immediate inhibition of RNase activity. Biological samples are homogenized or lysed in TRI Reagent™; the subsequent addition of bromochloropropane or chloroform results in the separation of the homogenate into aqueous and organic phases. RNA partitions to the aqueous phase, DNA to the interphase, and proteins to the organic phase. The RNA can then be precipitated from the aqueous phase with the addition of isopropanol. The entire protocol can be completed in approximately 1 hour.

Applications
The isolated RNA is suitable for any downstream application, including RT-PCR, amplification for array analysis, hybridization assays, or in vitro translation. PCR, Southern blotting, and cloning can be performed using DNA isolated with TRI Reagent™. Isolated proteins can be used in SDS-PAGE and western blotting.

Tri Reagent™ solution is incorporated into our RiboPure™ and MagMAX™-96 for Microarrays RNA isolation kits.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Elution VolumeVaries
Final Product TypeTotal RNA, DNA, Protein
For Use With (Application)RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Purification Time1 hr.
Quantity100 mL
Shipping ConditionRoom Temperature
Starting Material AmountBacteria: ≤107 cells
Cells: ≤106
Plant: ≤106 cells
Tissue: ≤100 mg
Yeast: ≤106 cells
YieldRNA: ≤15 μg (cells), ≤10 μg (tissue)
Isolation TechnologyOrganic Extraction
Sample TypeBacteria, Cells, Plants, Tissue, Yeast
Unit SizeEach
Contents & Storage
100 mL TRI Reagent; 4°C

Frequently asked questions (FAQs)

What type of sample can be used with TaqMan MicroRNA Assays?

One to ten nanograms of total RNA per 15 µL RT reaction is recommended for use with the TaqMan MicroRNA Assay. We have successfully detected miRNAs in as little as 25 pg of total RNA (~100 cells) in samples where the miRNA was highly abundant. The total RNA isolation procedure used must preserve small RNA species. We recommend the Invitrogen mirVana miRNA Isolation Kit (Cat. No. AM1560) or TRI reagent (Cat. No. AM9738).