Catalog number: AM9944

Ambion™  Related applications: Transformation

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Ambion® X-GAL (5-Bromo-4-Chloro-3 Indolyl-Beta-D-thiogalactopyranoside) is a chromogenic substrate for Beta-galactosidase that turns blue upon hydrolysis and is regularly used for the blue/white color selection assay. Bacteria with a lac+ phenotype grow as blue colonies on plates containing X-GAL. Supplied in one bottle containing 1 g. X-GAL is assayed for efficiency of color development of lac+ colonies and the absence of undesirable inhibitors of cell growth by testing a range of concentrations (recommended is 40 µg/mL) after plating a constant cell number of E. coli harboring pUC19 grown on LB/carbenicillin plates. The plates are evaluated for color intensity, size, and number of colonies after an overnight incubation at 37°C.
For Research Use Only. Not for use in diagnostic procedures.


Substrate: X-Gal
Product Size: 1 g
Substrate Type: Beta-Gal Substrate
Detection Method: Colorimetric
Cell Permeability: Cell-Permeant
Substrate Properties: Chemical Substrate
Shipping Condition: Room Temperature

Contents & storage

Store at –20°C.


Manuals & protocols

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