The EVOS Onstage Incubator is an environmental chamber designed specifically for the EVOS M5000 and EVOS M7000 imaging systems. It enables precise control of temperature, humidity, and three gases for time-lapse imaging of live cells under both physiological and non-physiological conditions. Environmental settings and image acquisition parameters are all seamlessly integrated into the microscope user interface, creating a high-performance inverted imaging system with excellent flexibility, ease of use, and a superb optical performance for demanding live cell time-lapse imaging experiments.

The EVOS Onstage Incubator offers you these important advantages:

• Fully integrated environmental chamber for live cell time-lapse imaging
• Precisely maintain physiological or non-physiological conditions
• Intuitively set acquisition parameters using the EVOS M5000 and M7000 interface
• Small footprint, robust build, and work-flow enhancing design elements
• Choice of a range of vessel holders

Fully Integrated Environmental Control System
The EVOS Onstage Incubator affords a complete solution for environmental control during live cell imaging studies. It consists of an environmental control unit and an environmental chamber that is precision-tooled for the automated X-Y stage on the EVOS M7000 system. The stage plate that ships with the Onstage Incubator is also compatible with EVOS M5000 system. A small, separate control unit supplies the power, gas (air or air-CO₂ premix, CO₂, and N₂ for O₂ displacement in hypoxia experiments), and control of humidity and temperature. Unlike many other systems that require separate software modules or control units, the EVOS Onstage Incubator is operated through the user interface of the EVOS M5000 and M7000 systems. Following a simple installation, the incubator is automatically recognized by the imaging software and is ready for use.

Flexible and Precise Environmental Control
When performing live cell imaging studies, such as cell proliferation, cell cycle, stem cell differentiation, migration, membrane, and organelle dynamics, it is essential to maintain environmental control. Shifts in temperature of even few degrees can have profound effects on dynamic cellular events, as can changes in CO₂ or oxygen concentrations. The ability to maintain tight temperature control helps maintain cell function and reduces the risk of biological temperature-related artifacts. It also minimizes thermal contractions or expansions that may cause focus instability or drift. To enable studies of cells under hypoxic conditions, the system has three gas line ports. By using N₂ gas it is possible to create hypoxia by setting the oxygen sensor to the desired level.

Designed for Live Cell Imaging
Many cells are highly sensitive to light and it is important to minimize light exposure that can cause photo-toxicity, particularly over the course of an extended time-lapse experiment; the digitally controlled light output from the proprietary EVOS light cubes enables light exposure to be minimized. By optimizing illumination intensity, the number of channels and autofocus point, and Z-steps and image acquisition intervals, it is possible to dramatically reduce photobleaching and phototoxic effects.

Explore the entire EVOS line of imaging systems and accessories.
Explore the entire collection of EVOS vessel holders and stage plates.

Specifications
Temperature range: ambient to 40°C (+/- 0.1°C)
CO² range: 0 to 20%
O² range: 0% to ambient
Humidity: >80% relative humidity (RH) at 37°C