EVOS™ Onstage Incubator

Catalog number: AMC1000

EVOS™ Onstage Incubator

Catalog number: AMC1000
Catalog Number
Unit Size
1 each
Price (USD)
Catalog NumberUnit SizePrice (USD)
AMC10001 eachRequest A Quote
Product Overview
Citations & References
Additional Information
The EVOS Onstage Incubator is an environmental chamber designed specifically for the EVOS M5000 and EVOS M7000 imaging systems. It enables precise control of temperature, humidity, and three gases for time-lapse imaging of live cells under both physiological and non-physiological conditions. Environmental settings and image acquisition parameters are all seamlessly integrated into the microscope user interface, creating a high-performance inverted imaging system with excellent flexibility, ease of use, and a superb optical performance for demanding live cell time-lapse imaging experiments.

The EVOS Onstage Incubator offers you these important advantages:

• Fully integrated environmental chamber for live cell time-lapse imaging
• Precisely maintain physiological or non-physiological conditions
• Intuitively set acquisition parameters using the EVOS M5000 and M7000 interface
• Small footprint, robust build, and work-flow enhancing design elements
• Choice of a range of vessel holders

Fully Integrated Environmental Control System
The EVOS Onstage Incubator affords a complete solution for environmental control during live cell imaging studies. It consists of an environmental control unit and an environmental chamber that is precision-tooled for the automated X-Y stage on the EVOS M7000 system. The stage plate that ships with the Onstage Incubator is also compatible with EVOS M5000 system. A small, separate control unit supplies the power, gas (air or air-CO2 premix, CO2, and N2 for O2 displacement in hypoxia experiments), and control of humidity and temperature. Unlike many other systems that require separate software modules or control units, the EVOS Onstage Incubator is operated through the user interface of the EVOS M5000 and M7000 systems. Following a simple installation, the incubator is automatically recognized by the imaging software and is ready for use.

Flexible and Precise Environmental Control
When performing live cell imaging studies, such as cell proliferation, cell cycle, stem cell differentiation, migration, membrane, and organelle dynamics, it is essential to maintain environmental control. Shifts in temperature of even few degrees can have profound effects on dynamic cellular events, as can changes in CO2 or oxygen concentrations. The ability to maintain tight temperature control helps maintain cell function and reduces the risk of biological temperature-related artifacts. It also minimizes thermal contractions or expansions that may cause focus instability or drift. To enable studies of cells under hypoxic conditions, the system has three gas line ports. By using N2 gas it is possible to create hypoxia by setting the oxygen sensor to the desired level.

Designed for Live Cell Imaging
Many cells are highly sensitive to light and it is important to minimize light exposure that can cause photo-toxicity, particularly over the course of an extended time-lapse experiment; the digitally controlled light output from the proprietary EVOS light cubes enables light exposure to be minimized. By optimizing illumination intensity, the number of channels and autofocus point, and Z-steps and image acquisition intervals, it is possible to dramatically reduce photobleaching and phototoxic effects.

Explore the entire EVOS line of imaging systems and accessories.
Explore the entire collection of EVOS vessel holders and stage plates.

Temperature range: ambient to 40°C (+/- 0.1°C)
CO2 range: 0 to 20%
O2 range: 0% to ambient
Humidity: >80% relative humidity (RH) at 37°C
For Research Use Only. Not for use in diagnostic procedures.


Onstage Incubator
Product Line
For Use With (Equipment)
EVOS FL Auto 2 (AMAFD2000), EVOS M5000 (AMF5000)
Chamber Slides, T-25 Flasks, Microscope Slides, Multi-well Plates, Petri Dishes
Unit includes:
• Stagetop environmental chamber
• Control unit
• Master stage plate (also available as EVOS Onstage Master Plate, Cat. No. AMEPVH035)
• Vessel holder for multi-well plates (also available as EVOS Onstage Vessel Holder, Multiwell Plates, Cat. No. AMEPVH028)
• Lid retainer shield
• Cable with 6-pin connector
• Cable, USB A-to-B, 6 ft
• Heated hose with temperature control, 180/6 (cm, ft) (also available as EVOS Onstage Incubator Hose, Cat. No. AMEP4728)
• Gas line, 1/8' ID, 1/4' OD (also available as EVOS Onstage Incubator Gas Line, Cat. No. AMEP4732)
• Push-to-connect gas line adapter (3 ea)
• Standard-head open-end wrench
• Hex screw driver
• Power Cord, Type A (North America)*

*Note that a country-specific power cord must be ordered separately in regions not using the Type A power plug.
Light Source
No Lighting
Relative Humidity
<80% at 37°C
Temperature Range (Metric)
Ambient to 40°C
Control unit (HxWxD): 37 x 16 x 20 cm (15 x 6.3 x 7.9 in.), Environmental chamber (HxWxD): 25 x 19 x 3.7 cm (9.7 x 7.6 x 1.5 in.)
Weight (English)
Control unit: 10 kg (22 lb), Environmental chamber: 1.5 kg (3.3 lb)