Countess® II FL Automated Cell Counter This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: AMQAF1000

 Related applications: Cell Counting, Viability, & Cryopreservation

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The new Countess™ II FL Automated Cell Counter is a benchtop assay platform equipped with state-of-the-art optics, full autofocus, and image analysis software for rapid assessment of cells in suspension. With three-channel flexibility—brightfield and two optional fluorescence channels—researchers can count cells, monitor fluorescent protein expression, evaluate apoptosis, and measure cell viability.

Accurate—use the Countess II FL Automated Cell Counter to eliminate the subjectivity of manual cell counting and user-to-user variability
Flexible—the Countess II FL Automated Cell Counter is able to accept up to two EVOS® light cubes that are user-selectable from over twenty fluorescence channels
Fast—the Countess II FL Automated Cell Counter provides a total cell count, reports viability, and measures average cell size in as little as 10 seconds. Counting speed is identical when using fluorescence channels.
Convenient—the Countess II FL Automated Cell Counter requires no cleaning or routine maintenance. A capacitive touchscreen and simple user interface provides quick startup and requires minimal training.

Learn more about our automated cell counters >

The Countess II FL Automated Cell Counter comes with:
• Countess USB drive for storing and transferring data to your computer
• Disposable slide holder
• Reusable slide holder
• Light cube removal tool
• Power cord and 4 plug adaptors to accommodate most countries
• Quick reference card (QRC)
• A free box of 50 disposable slides

The Countess II FL Automated Cell Counter works in 3 simple steps:
1. Mix 10 µL of sample with 10 µL of trypan blue and pipet into a disposable Countess chamber slide.
2. Insert the slide into the instrument.
3. Press the "Count" button.

Extended Warranty Packages
Protect your instrument and your investment with the Countess II FL Automated Cell Counter Extended Warranty Packages. Save up to 25% off the individual instrument and extended warranty list prices when purchased together. Learn more about the packages >

Get accurate cell counts
The Countess II FL Automated Cell Counter uses trypan blue staining combined with an auto-focus mechanism and sophisticated image analysis algorithm to obtain accurate cell and viability counts. The measurement range extends from 1 x 10e4 to 1 x 10e7 cells/mL (the optimal range is 1 x 10e5 to 4 x 10e6 cells/mL), which is broader than that of a hemocytometer. The optimal cell size for accurate viability assessment is 7–60 µm, while particles as small as 4 µm can be successfully counted.

Simple operation with fluorescent application versatility
Operated using an intuitive user interface, automated cell counting with the Countess II FL instrument gives you the data you need to prepare your sample for the next passage or experiment, and allows you to multiplex with a variety of fluorescent dyes and proteins excited via EVOS® Light Cubes.

Transfer data and images to your PC
Using the USB port on the Countess II FL Automated Cell Counter, you can save your cell count results and images, and then transfer them to your PC. (For your convenience, a USB drive is supplied with the Countess II FL instrument).

Countess II/II FL Software >

For Research Use Only. Not for use in diagnostic procedures.


Field: 2.15 mm x 1.62 mm (3.48 mm2)
Weight: 8 lbs
Capacity: One 2-chamber disposable or 2-chamber reusable slide
Cell Size: 4-60 µm (detection), 7-60 µm (viability)
Dimensions: 9 in (228.6 mm) W x 5.5 in (139.7 mm) D x 9 in (228.6 mm) W
Product Size: 1 instrument
Concentration: 1x10^4-1x10^7 cells⁄mL
Input Voltage: 100-240 VAC
Green Features: Fewer resources used, Less waste
Detection Method: Brightfield & 2 user-definable FL channels
Display Interface: 7" Capacitive touch screen
Instrument Memory: USB Stick
Shipping Condition: Room Temperature

Contents & storage

• Countess II FL Automated Cell Counter
• Power cord with adaptors
• Quick reference guide
• USB drive
• Disposable Slide Holder
• Reusable Slide Holder


Manuals & protocols