Thermo Scientific™

Lab Vision™ Pepsin Solution for Enzyme-Induced Epitope Retrieval

 Related applications:
Anatomical Pathology
Improve the immunoreactivity of many antigens in formaldehyde fixed tissues with Thermo Scientific™ Lab Vision™ Pepsin Solution for Enzyme-Induced Epitope Retrieval.

Error loading your content!

  Catalog number Price ({{currency}}) Availability Qty
{{product.sku}}
also known as {{product.formattedSku}} 
{{product.availability.message}} Add To Cart

{{addItemToList.successMessage}} {{addItemToList.listName}}


Back to top

Description

  • pH 2.0
  • Single-component, ready-to-use format

Storage Conditions: Store at 4°C. Stable for 12 months when stored at 2° to 8°C.
Procedure:

1. Place five-micron thick tissue sections on glass slides coated with poly L-lysine or APTES.

2. Deparaffinize and rehydrate sections as usual.

3. Block endogenous peroxidase as usual.

4. Wash sections in wash-buffer for 2 x 5 minutes.

5. Cover sections with the pepsin solution (usually 0.2mL per section) and place the slides at 37°C for 10 minutes in a humidified chamber.

6. Wash sections in wash buffer for 2 x 5 minutes.

7. Block nonspecific sites with normal serum as usual.

8. Place optimally diluted primary antibody on the sections (incubation time and temperature for a given set of experimental conditions should be determined by the investigator).

9. Wash sections in buffer for 2 x 5 minutes.

10. Remaining procedure is same as routinely performed in your laboratory.



Documents

Manuals & protocols