Aluora™ Spatial Amplification Individual Dyes
Aluora™ Spatial Amplification Individual Dyes
Invitrogen™

Aluora™ Spatial Amplification Individual Dyes

Invitrogen Aluora spatial amplification individual dyes provide a highly sensitive method for multiplex immunohistochemical (mIHC) analysis for use in HRP-mediated catalyzed covalent deposition.
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Catalog NumberConjugateExcitation/Emission
AS750HRPAluora 750757/783 nm
AS430HRPAluora 430427/499 nm
AS488HRPAluora 488493/518 nm
AS514HRPAluora 514512/529 nm
AS555HRPAluora 555553/567 nm
AS594HRPAluora 594589/615 nm
AS647HRPAluora 647652/670 nm
AS700HRPAluora 700687/706 nm
8 Options
Catalog number AS750HRP
Price (USD)/ 100 reactions
695.00
-
Add to cart
Conjugate:
Aluora 750
Excitation/Emission:
757/783 nm
Price (USD)/ 100 reactions
695.00
Add to cart
Aluora™ Spatial Amplification Individual Dyes
Catalog numberAS750HRP
Price (USD)/ 100 reactions
695.00
-
Add to cart
Invitrogen Aluora spatial amplification individual dyes provide a highly sensitive method for multiplex immunohistochemical (mIHC) analysis for use in HRP-mediated catalyzed covalent deposition. These dyes enable up to 8-plex fluorescent labeling with precision and sensitivity 10–200X greater than standard IHC and 2–10X that of other tyramide and CARD techniques.

Revolutionary signal amplification technology

The Aluora spatial amplification kits represent a breakthrough in signal amplification for spatial proteomics. They employ proprietary Aluora reagents that react with horseradish peroxidase (HRP) to deposit photostable Aluora dyes onto target proteins, significantly improving the signal-to-background ratio. This technology enables robust multiplexed protein detection even in challenging tissue samples. Aluora kits require 10–5000X less primary antibody than standard IHC to achieve equivalent signal intensity.

Aluora spatial amplification reagents are optimized for use with Prolong Glass Antifade mountants and 1 mg/mL Thermo Scientific DAPI nucleic acid stains for multiplex analysis.

Aluora reagents and dyes can be purchased individually or in pre-configured kits to detect mouse, rabbit, or biotin-labelled antibodies. The Rainbow kit is configured with all Aluora colors with 4X GAM and 4X GAR secondary detection core reagents for 100 slides in 8-plex.

Features of Aluora spatial amplification reagents include:

  • Signal amplification for enhanced detection sensitivity
  • Comprehensive spatial analysis and imaging capabilities for research
  • Fluorescent detection system for multiplexing and quantitative analysis
  • Flexible workflow options that accommodate diverse experimental needs
  • Polymerase HRP (PolyHRP) conjugated secondary antibodies that boost signal for low abundance targets
  • Highly cross-adsorbed secondary antibodies that help ensure target-specific detection and background reduction
  • Enhanced background reduction through proprietary blocking reagents for crisp, clear imaging

Optimized for automated slide staining

A major benefit of Aluora spatial amplification kits is their compatibility with automated slide stainers right out of the box. This enables complete labeling of a tissue slide with an 8-plex Aluora panel in just 12–16 hours.

Eliminating background for cleaner images

Aluora spatial amplification kits include blockers to help reduce endogenous peroxidase activity and autofluorescence. By minimizing background noise, less primary antibody is needed to generate a strong specific signal.

Iterative labeling enables unlimited multiplexing

The covalently bound Aluora dyes are resistant to antibody stripping, remaining brightly fluorescent through subsequent labeling cycles. This enables iterative staining of numerous targets using primary antibodies from the same host species—a major constraint in traditional immunohistochemistry. Any number of directly conjugated primary antibodies can also be introduced after Aluora labeling is complete.

Custom optimization for diverse applications

Although general protocols are provided, each sample requires optimization for optimal results. Tissue preparation and processing methods vary widely and can profoundly impact staining. Careful optimization of the complete workflow is critical to achieve the highest quality multiplexed data from precious samples. Aluora spatial amplification provides a versatile tool to address diverse spatial proteomics applications.

For Research Use Only.
Specifications
Product TypeSpatial Amplification Dye
Quantity100 Reactions
For Use With (Application)Spatial Proteomics, Immunohistochemistry (IHC), IHC Staining and Detection, Fluorescent Staining, Immunofluorescence (IF)
Product LineAluora™
ConjugateAluora 750
Detection MethodFluorescence
Sample TypeFFPE Tissue Sections
Sufficient ForSpatial Proteomics
Excitation/Emission757/783 nm
Unit Size100 reactions