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20X Bolt™ MOPS SDS Running Buffer, 5 L - FAQs

20X Bolt™ MOPS SDS Running Buffer, 5 L - FAQs

View additional product information for 20X Bolt™ MOPS SDS Running Buffer - FAQs (B0001, B000102)

8 product FAQs found

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Which power supplies are compatible with the Mini Gel Tank?

The most common power supplies from Thermo Fisher Scientific, Bio-Rad, and Hoefer are compatible. Also, power supply adapters are available for power supplies not designed for use with covered or non-retractable power leads. Thermo Fisher Owl branded gel tanks are not compatible

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Are Bolt gels compatible with transfer devices from other suppliers?

Yes. While we would prefer that you use our devices, Bolt gels can also be transferred using devices from Bio-Rad, including the Mini Trans-Blot Cell, Trans-Blot SD Semi-Dry Transfer Cell, or Trans-Blot Turbo Transfer System.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Are Bolt gels compatible with other transfer devices?

Yes. You can use the iBlot Dry Transfer System or XCell II Blot Module with SureLock tank and achieve similar transfer efficiency.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.