D-Biotina: la d-biotina se une con alta afinidad a las proteínas de estreptavidina, avidina y Neutravidin™ de afinidad a laMás información
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Número de catálogo
Cantidad
B20656
10 ml
B1595
1 g
2 opciones
Número de catálogo B20656
Precio (MXN)
-
Cantidad:
10 ml
D-Biotina: la d-biotina se une con alta afinidad a las proteínas de estreptavidina, avidina y Neutravidin™ de afinidad a la biotina.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración50mM
Tipo de productoBiotina (no conjugada)
Cantidad10 ml
Condiciones de envíoTemperatura ambiente
Conjugadono conjugado
FormularioLíquido
Línea de productosInvitrogen
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador (2–8° C).
Preguntas frecuentes
How should I resolubilize D-biotin (B20656)?
D-biotin may be resolubilized in DMF (1-2 mg/mL), DMSO (49 mg/mL), ethanol (<1 mg/mL) or a buffer with a pH between >6 and <9. For long-term stability, D-biotin is stable in aqueous buffers at moderately acidic to neutral pH.
Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.
Authors:Hirsch JD, Eslamizar L, Filanoski BJ, Malekzadeh N, Haugland RP, Beechem JM, Haugland RP
Journal:Anal Biochem
PubMed ID:12419349
'The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades. However, a disadvantage of the biotin/biotin-binding protein interaction is that it is essentially irreversible under physiological conditions. Desthiobiotin is a biotin analogue that binds less tightly to biotin-binding proteins and is easily displaced by ... More
A SPECTROPHOTOMETRIC ASSAY FOR AVIDIN AND BIOTIN BASED ON BINDING OF DYES BY AVIDIN.
Authors:GREEN NM,
Journal:Biochem J
PubMed ID:14340040
A spectrophotometric assay for avidin and biotin based on binding of dyes by avidin.