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Thermo Scientific Chemicals
Isopropyl-beta-D-thiogalactoside, dioxane-free, 99%, Thermo Scientific Chemicals
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| Catalog Number | Quantity |
|---|---|
| B21149.03 also known as B21149-03 | 1g |
| B21149.06 also known as B21149-06 | 5g |
Chemical Identifiers
CAS367-93-1
IUPAC Name(2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(propan-2-ylsulfanyl)oxane-3,4,5-triol
Molecular FormulaC9H18O5S
InChI KeyBPHPUYQFMNQIOC-NXRLNHOXSA-N
SMILESCC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O
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Specifications
Specification Sheet
Specification SheetFormPowder or crystalline powder
Assay (Silylated GC)≥98.5% (non-U.S. specification)
Assay from Suppliers CofA≥98.0% (HPLC: U.S. specification)
CommentSpecification differs for U.S. and non-U.S. material where indicated
Melting Point109.5-115.5°C
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Beta-galactosidase inducer.Isopropyl-β-D-thiogalactoside is used as a reagent in molecular biology. It is used as an effective inducer of protein expression where the gene is under the control of the lac operator. It is used with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside to identify the non-recombinant plasmid in cloning experiments by blue-white screen methodology.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Beta-galactosidase inducer.Isopropyl-β-D-thiogalactoside is used as a reagent in molecular biology. It is used as an effective inducer of protein expression where the gene is under the control of the lac operator. It is used with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside to identify the non-recombinant plasmid in cloning experiments by blue-white screen methodology.
Solubility
Soluble in water.
Notes
Hygroscopic. Store in a cool place. Incompatible with strong oxidizing agents.
Beta-galactosidase inducer.Isopropyl-β-D-thiogalactoside is used as a reagent in molecular biology. It is used as an effective inducer of protein expression where the gene is under the control of the lac operator. It is used with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside to identify the non-recombinant plasmid in cloning experiments by blue-white screen methodology.
Solubility
Soluble in water.
Notes
Hygroscopic. Store in a cool place. Incompatible with strong oxidizing agents.
RUO – Research Use Only
Kordafshari, S.; Hosseini, S. H.; Jalousian, F.; Rajabibazl, M.; Jones, M.; Etebar, F. Evaluation of Dot Immunogold Filtration Assay (DIGFA) By Recombinant Protein EPC1 for Anti-Echinococcus granulosus IgG Antibody. Iran J Parasitol 2015, 10 (1), 30-38.
He, Y.; Hou, P.; Fan, G.; Li, D.; Peng, C. Isolation and characterization of a novel (S)-canadine synthase gene from Coptis chinensis. Electron. J. Biotechnol. 2015, 18 (5), 376-380.
He, Y.; Hou, P.; Fan, G.; Li, D.; Peng, C. Isolation and characterization of a novel (S)-canadine synthase gene from Coptis chinensis. Electron. J. Biotechnol. 2015, 18 (5), 376-380.