Nitro Blue Tetrazolium chloride, tech. 90%

CAS: 298-83-9 | C40H30Cl2N10O6 | 817.644 g/mol

Catalog Number	Quantity
B23792.02 also known as B23792-02	0.5 g

Catalog number B23792.02

also known as B23792-02

Price (USD)

93.65

Online Exclusive

104.00

Save 10.35 (10%)

Quantity:

0.5 g

Request bulk or custom format

Price (USD)

93.65

Online Exclusive

104.00

Save 10.35 (10%)

Chemical Identifiers

CAS298-83-9

IUPAC Name2-{3,3'-dimethoxy-4'-[3-(4-nitrophenyl)-5-phenyl-3H-1,2λ⁵,3,4-tetrazol-2-ylium-2-yl]-[1,1'-biphenyl]-4-yl}-3-(4-nitrophenyl)-5-phenyl-3H-1,2λ⁵,3,4-tetrazol-2-ylium dichloride

Molecular FormulaC40H30Cl2N10O6

InChI KeyFSVCQIDHPKZJSO-UHFFFAOYSA-L

SMILES[Cl-].[Cl-].COC1=CC(=CC=C1[N+]1=NC(=NN1C1=CC=C(C=C1)[N+]([O-])=O)C1=CC=CC=C1)C1=CC=C(C(OC)=C1)[N+]1=NC(=NN1C1=CC=C(C=C1)[N+]([O-])=O)C1=CC=CC=C1

Specifications

Appearance (Color)Yellow

FormPowder

Assay (Titration ex Chloride)≥88.0%

Loss on Drying<12.0% (105°C/constant wt.)

Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease. It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase. Further, it is used in immunology for detection of alkaline phosphatase. In addition to this, it acts as an oxidant.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease. It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that

RUO – Research Use Only

General References:

Substrate for determination of various enzymes.
Materska, M.; Konopacka, M.; Rogoliński, J.; Ślosarek, K. Antioxidant activity and protective effects against oxidative damage of human cells induced by X-radiation of phenolic glycosides isolated from pepper fruits Capsicum annuum L. Food Chem. 2015, 168, 546-553.
Xiong, B.; Xu, R.; Zhou, R.; He, Y.; Yeung, E. S. Preventing UV induced cell damage by scavenging reactive oxygen species with enzyme-mimic Au-Pt nanocomposites. Talanta 2014, 120, 262-267.