BacLight™ RedoxSensor™ Green Vitality Kit

<i>Bac</i>Light™ RedoxSensor™ Green Vitality Kit

Citas y referencias (8)

Invitrogen™

BacLight™ RedoxSensor™ Green Vitality Kit

El reactivo verde RedoxSensor™ incluido en el kit de vitalidad Bac Light™ RedoxSensor™ Green es un indicador de la actividadMás información

Número de catálogo	Cantidad
B34954	200 kits

Número de catálogo B34954

Precio (MXN)

Cantidad:

200 kits

El reactivo verde RedoxSensor™ incluido en el kit de vitalidad Bac Light™ RedoxSensor™ Green es un indicador de la actividad de la reductasa bacteriana. Esta actividad de la reductasa es, a su vez, un marcador fiable para los cambios en la función de la cadena de transporte de electrones y para los cambios en la vitalidad que ocurren después del tratamiento con antibióticos. El reactivo RedoxSensor™ Green penetra en bacterias grampositivas y gramnegativas. Tras la reducción, el reactivo RedoxSensor™ Green producirá una señal verde fluorescente estable en 10 minutos compatible con las técnicas de fijación con formaldehído.

Consulte información adicional acerca de todos los ensayos microbiológicos para citometría de flujo.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tipo de célulaBacterias

Método de detecciónFluorescente

Tipo de coloranteRedoxSensor™ Green, PI

FormularioSolución

FormatoTubos, portaobjetos

Cantidad200 kits

Condiciones de envíoTemperatura ambiente

SolubilidadDMSO (dimetilsulfóxido)

EmissionPI: 490⁄635, RedoxSensor™ Green: 490⁄520

Para utilizar con (equipo)Microscopio de fluorescencia, Citómetro de flujo

Línea de productosBacLight, RedoxSensor

Tipo de productoKit de vitalidad verde

Unit SizeEach

Contenido y almacenamiento

Contiene un vial de RedoxSensor™ Green (200 μl, solución de 1 mm en DMSO), un vial de yoduro de propidio (300 μl, solución de 20 mm en DMSO), un vial de azida sódica (1 ml de una solución de 2 M en agua) y un vial de CCCP (400 μl, 5 mm en DMSO).

Preguntas frecuentes

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the excitation/emission of the RedoxSensor Green reagent?

The excitation/emission of the RedoxSensor Green reagent, in reduced form, is 490/520 nm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (8)

Citations & References

Abstract

Respiration response imaging for real-time detection of microbial function at the single-cell level.

Authors:Konopka MC, Strovas TJ, Ojala DS, Chistoserdova L, Lidstrom ME, Kalyuzhnaya MG,

Journal:Appl Environ Microbiol

PubMed ID:21075887

'The ability to detect specific functions of uncultured microbial cells in complex natural communities remains one of the most difficult tasks of environmental microbiology. Here we present respiration response imaging (RRI) as a novel fluorescence microscopy-based approach for the identification of microbial function, such as the ability to use C(1) ... More

Extracellular reduction of uranium via Geobacter conductive pili as a protective cellular mechanism.

Authors:Cologgi DL, Lampa-Pastirk S, Speers AM, Kelly SD, Reguera G,

Journal:Proc Natl Acad Sci U S A

PubMed ID:21896750

'The in situ stimulation of Fe(III) oxide reduction by Geobacter bacteria leads to the concomitant precipitation of hexavalent uranium [U(VI)] from groundwater. Despite its promise for the bioremediation of uranium contaminants, the biological mechanism behind this reaction remains elusive. Because Fe(III) oxide reduction requires the expression of Geobacter''s conductive pili, ... More

Fullerene water suspension (nC60) exerts antibacterial effects via ROS-independent protein oxidation.

Authors:Lyon DY, Alvarez PJ,

Journal:Environ Sci Technol

PubMed ID:19031913

Buckminsterfullerene (C60) can form water suspensions (nC60) that exert toxic effects. While reactive oxygen species (ROS) generation has been implicated as the mechanism for mammalian cytotoxicity, we propose that nC60 exerts ROS-independent oxidative stress in bacteria, with evidence of protein oxidation, changes in cell membrane potential, and interruption of cellular ... More

Oxygen consumption rates of bacteria under nutrient-limited conditions.

Authors:Riedel TE, Berelson WM, Nealson KH, Finkel SE,

Journal:

PubMed ID:23770901

Many environments on Earth experience nutrient limitation and as a result have nongrowing or very slowly growing bacterial populations. To better understand bacterial respiration under environmentally relevant conditions, the effect of nutrient limitation on respiration rates of heterotrophic bacteria was measured. The oxygen consumption and population density of batch cultures ... More

In vitro susceptibility and cellular uptake for a new class of antimicrobial agents: dinuclear ruthenium(II) complexes.

Authors:Li F, Feterl M, Mulyana Y, Warner JM, Collins JG, Keene FR,

Journal:J Antimicrob Chemother

PubMed ID:22865383

To determine the in vitro susceptibility and cellular uptake for a series of dinuclear ruthenium(II) complexes [{Ru(phen)(2)}(2){µ-bb(n)}](4+) (Rubb(n)), and the mononuclear complexes [Ru(Me(4)phen)(3)](2+) and [Ru(phen)(2)(bb(7))](2+) against Staphylococcus aureus, methicillin-resistant S. aureus, Escherichia coli and Pseudomonas aeruginosa. The in vitro susceptibility was determined by MIC and MBC assays, and time-kill curve ... More

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