The RedoxSensor™ Green reagent included in the BacLight™ RedoxSensor™ Green Vitality Kit is an indicator of bacterial reductase activity. This reductase activity is, in turn, a reliable marker for changes in electron transport chain function and for changes in vitality that occurs following antibiotic treatment. RedoxSensor™ Green reagent penetrates both gram-positive and gram-negative bacteria. Following reduction, the RedoxSensor™ Green reagent will produce a stable green-fluorescent signal in 10 minutes that is compatible with formaldehyde fixation techniques.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell Type
Bacteria
Detection Method
Fluorescent
For Use With (Equipment)
Fluorescence Microscope, Flow Cytometer
Product Type
Green Vitality Kit
Dye Type
RedoxSensor™ green, PI
Emission
PI: 490⁄635, RedoxSensor™ green: 490⁄520
Form
Solution
Format
Tube(s), Slide(s)
Product Line
BacLight™, RedoxSensor™
Quantity
200
Shipping Condition
Room Temperature
Solubility
DMSO (Dimethylsulfoxide)
Contents & Storage
Contains 1 vial of RedoxSensor™ green (200 μL, 1 mM solution in DMSO), 1 vial of propidium iodide (300 μL, 20 mM solution in DMSO), 1 vial of sodium azide (1 mL of a 2 M solution in water), and 1 vial of CCCP (400 μL, 5mM in DMSO).