Alexa Fluor™ 555 Tyramide SuperBoost™ Kit, streptavidin

Alexa Fluor™ 555 Tyramide SuperBoost™ Kit, streptavidin - FAQs

View additional product information for Alexa Fluor™ 555 Tyramide SuperBoost™ Kit, streptavidin - FAQs (B40933)

3 product FAQs found

With a SuperBoost tyramide kit, I got excessive and non-specific labeling. What can I do to limit background and acquire a more localized labeling?

To limit background, we recommend performing a pre-blocking step with 3% H2O2 for 60 mins to inactivate endogenous peroxidases. To limit the localization of labeling, we recommend optimizing the final concentration of the primary and secondary antibodies and the dye-tyramide. You may also limit the incubation time of the dye-tyramide on the sample.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is it possible to perform dual TSA labeling with SuperBoost tyramide kits?

Yes. This involves the sequential application of the antibodies and the tyramides with a HRP-quenching step between antibodies using H2O2.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How are SuperBoost tyramide kits different from the original TSA labeling kits?

The SuperBoost tyramide kits utilize poly-HRP labeled antibodies. This provides a greater number of horseradish peroxidase (HRP) molecules per antibody. The original kits used antibodies and streptavidin that were directly conjugated with HRP and thus, limited the number per antibody or streptavidin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.