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Invitrogen™

Alexa Fluor™ 488 Tyramide Reagent

Catalog number:  B40953

 Related applications:

Cellular Imaging

| Secondary Detection

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Description

Alexa Fluor™ 488 Tyramide Reagent is a component of SuperBoost™ tyramide signal amplification kits made available separately here. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

SuperBoost reagents, when used as directed, can increase sensitivity up to 200 times over standard imaging methods. For standout research, SuperBoost reagents sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal. SuperBoost reagents are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost reagent can be imaged using any type of microscope, producing high-resolution multiplex images. Alexa Fluor™ 488 Tyramide Reagent (495/519 ex/em) can be detected using a standard Green/FITC/GFP filter cube.

Features of the SuperBoost reagents include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with TO-PRO™-3, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: Alexa Fluor™ 488
Product Line: SuperBoost™
Product Size: 150 slides
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

1 vial Alexa Fluor Tyramide Reagent sufficient for 150 slides
Store at 2-8°C. When stored as directed, the product is stable for 6 months after receiving.

Documents

Manuals & protocols