Invitrogen™

eBioscience™ Propidium Iodide

Catalog number:  BMS500PI

Related applications:

Flow Cytometry

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Description

Propidium Iodide (PI) is a standard reagent used for assessing cell viability and exclusion of non-viable cells in flow cytometry. PI binds to double stranded DNA, but is excluded from cells with intact plasma membranes. PI should be analyzed in the PE channel when used as a counterstain for Annexin V FITC. 7-AAD can be used in place of PI when using Annexin V PE. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions. Choose propidium Iodide as a counterstain with Annexin V apoptosis assays.

Reactivity/Species
Human, Mouse, Rat

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Permeability: Cell-Impermeant
Detection Method: Fluorescent
Excitation/Emission: 535⁄617
For Use With (Equipment): Flow Cytometer
Product Line: eBioscience™
Color: Red