Image-iT™ Lipid Peroxidation Kit, for live cell analysis

Description

The Image-iT® Lipid Peroxidation Kit enables the detection of lipid peroxidation in live cells through oxidation of BODIPY® 581/591 C11 reagent. This reagent localizes to membranes throughout live cells and upon oxidation by lipid hydroperoxides, displays a shift in peak fluorescence emission from ~590 nm to ~510 nm. Fluorescence from live cells shifts from red to green, providing a ratiometric indication of lipid peroxidation compatible with traditional and high content microscopy, as well as flow cytometry. This kit also includes cumene hydroperoxide as a positive control compound to induce lipid peroxidation in cells.

Additional Information:

• The reagent is provided as a stable, ready-to-use DMSO solution with a simple protocol compatible with standard workflows in fluorescence microscopy
• The reagent is provided as five single-use vials containing sufficient total reagent for five 96-well plates or 100 coverslips

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Figures

Specifications

Color:	Green
Detection Method:	Fluorescent
Form:	Frozen
For Use With (Equipment):	Floid™ Cell Imaging System, Fluorescence Microscope, Flow Cytometer, High Content Instrument
Indicator:	Lipid Peroxidation Sensor
Solubility:	DMSO (Dimethylsulfoxide)
Sub Cellular Localization:	Cytoplasm
Shipping Condition:	Wet Ice
Product Line:	Image-iT™

Contents & storage