Invitrogen™

CellROX™ Deep Red Flow Cytometry Assay Kit

Catalog number:  C10491

Related applications:

Cell Viability, Proliferation & Function

| Flow Cytometry

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Description

The CellROX™ Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Deep Red Reagent, as well as SYTOX™ Blue dead cell stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).

View a selection guide for all CellROX® Reagents and Kits.

The CellROX™ Deep Red Flow Cytometry Assay Kit features:

• Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS
• Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing easy multiplexing with other reagents
• Simple protocol—cells can be stained in complete media or other appropriate buffer, no need for serum free media

The CellROX™ Deep Red Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent exhibits a strong fluorogenic signal that has absorption/emission maxima of 644/665 nm and remains localized in the cytoplasm. When used together with the included SYTOX™ Blue Dead Cell Stain, oxidatively stressed and non-stressed cells are reliably distinguished from dead cells by flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Fluorescent
Format: Tube(s)
Compatible Cells: Mammalian Cells, Eukaryotic Cells
Flow Cytometer Laser Lines: 633/635, UV or 405
Excitation/Emission: CellROX® Deep Red: 644⁄665, SYTOX® blue: 444⁄480
For Use With (Equipment): Flow Cytometer
Label or Dye: CellROX® Deep Red Reagent
No. of Reactions: 100
Solubility: DMSO (Dimethylsulfoxide)
Shipping Condition: Wet Ice
Product Line: CellROX®

Contents & storage

Contains 1 vial CellROX® Deep Red Reagent (25 µL), 1 vial SYTOX® Blue Dead Cell Stain (100 µL), 2 vials N-acetylcysteine (10 mg per vial), 1 vial tert-butyl hydroperoxide (50 µL, 70% solution in water), and 1 vial DMSO (200 µL).

Store kit at -5 to -30°C, protected from light. CellROX® reagent is air sensitive.