Invitrogen™

CellROX™ Deep Red Flow Cytometry Assay Kit

Catalog number: C10491
Invitrogen™

CellROX™ Deep Red Flow Cytometry Assay Kit

Catalog number: C10491
Catalog Number
C10491
Unit Size
100 assays
Price (USD)
376.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C10491100 assays
376.00
Your Price:
***
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The CellROX™ Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Deep Red Reagent, as well as SYTOX™ Blue dead cell stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).

View a selection guide for all CellROX® Reagents and Kits.

The CellROX™ Deep Red Flow Cytometry Assay Kit features:

• Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS
• Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing easy multiplexing with other reagents
• Simple protocol—cells can be stained in complete media or other appropriate buffer, no need for serum free media

The CellROX™ Deep Red Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent exhibits a strong fluorogenic signal that has absorption/emission maxima of 644/665 nm and remains localized in the cytoplasm. When used together with the included SYTOX™ Blue Dead Cell Stain, oxidatively stressed and non-stressed cells are reliably distinguished from dead cells by flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
Mammalian Cells, Eukaryotic Cells
Detection Method
Fluorescent
Dye Type
CellROX™ Deep Red Reagent
Emission
CellROX™ Deep Red: 644⁄665, SYTOX™ blue: 444⁄480
Format
Tube(s)
Product Line
CellROX™
Quantity
100
Shipping Condition
Wet Ice
Solubility
DMSO (Dimethylsulfoxide)
For Use With (Equipment)
Flow Cytometer

Contents & Storage

Contains 1 vial CellROX™ Deep Red Reagent (25 µL), 1 vial SYTOX™ Blue Dead Cell Stain (100 µL), 2 vials N-acetylcysteine (10 mg per vial), 1 vial tert-butyl hydroperoxide (50 µL, 70% solution in water), and 1 vial DMSO (200 µL).

Store kit at -5 to -30°C, protected from light. CellROX™ reagent is air sensitive.

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