Invitrogen™

CellLight™ Lysosomes-RFP, BacMam 2.0

Catalog number: C10504
Invitrogen™

CellLight™ Lysosomes-RFP, BacMam 2.0

Catalog number: C10504
Catalog Number
C10504
Unit Size
100 µL
Price (USD)
Price: 128.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1 mL
Price (USD)
Price: 558.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C10504100 µL
Price: 128.00
Your Price:
***
C105971 mL
Price: 558.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
CellLight™ Lysosomes-RFP, BacMam 2.0, provides an easy way to label lysosomes with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight™ fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to lamp1 (lysosomal associated membrane protein 1). You can observe lysosomes-RFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight™ constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight™ Technology is:
Fast and convenient: simply add CellLight™ reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight™ reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight™ Lysosomes-RFP, BacMam 2.0, is a fusion construct of Lamp1 (lysosomal associated membrane protein 1) and TagRFP, providing accurate and specific targeting to cellular lysosomes-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight™ reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color
Red-Orange
Excitation Wavelength Range
555⁄584
Dye Type
RFP (TagRFP)
For Use With (Equipment)
Confocal Microscope, Floid™ Cell Imaging System, Fluorescence Microscope, Microscope
Quantity
100μL
Detection Method
Fluorescent
Delivery Type
Baculovirus (BacMam System)
Emission
Visible
Form
Liquid
Product Line
CellLight™
Shipping Condition
Wet Ice
Technique
In Vivo Imaging, Fluorescence Intensity
Label Type
Fluorescent Protein
Product Type
Lysosome
SubCellular Localization
Lysosomes

Contents & Storage

Store at 2°C to 6°C, protected from light. Do Not Freeze.

Figures