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CellLight® Mitochondria-GFP, BacMam 2.0

Catalog number: C10508

Molecular Probes™  Related applications: Cell Structure | Cell Viability, Proliferation & Function | Cellular Imaging

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Description

CellLight® Mitochondria-GFP, BacMam 2.0, provides an easy method for the labeling of mitochondria with Green Fluorescent Protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning. This ready-to-use construct is transduced/transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to the Leader sequence of E1 alpha pyruvate dehydrogenase. You can observe mitochondria-GFP behavior in live cells using a fluorescent imaging system and standard FITC/GFP filter set. Study staining of mitochondria, which does not depend upon mitochondrial membrane potential. In addition, you can co-transduce/transfect more than one CellLight® reagent and label live cells with multiple tracking or tracing dyes to image dynamic cellular processes. Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexing with antibodies using immunocytochemical techniques.

CellLight® Technology
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose

Learn more about CellLight® fluorescent protein labeling

BacMam Technology
CellLight® Mitochondria-GFP, BacMam 2.0, is a fusion construct of the Leader sequence of E1 alpha pyruvate dehydrogenase and emGFP, providing accurate and specific targeting to cellular mitochondria-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color: Green
Delivery Method: Baculovirus (BacMam System)
Detection Method: Fluorescent
Emission Class: Visible
Excitation Class: Visible
Excitation⁄Emission (nm): 488⁄510
Flow Cytometer Laser Lines: 488
For Use With (Equipment): Attune® Acoustic Focusing Cytometer, Confocal Microscope, Floid® Cell Imaging System, Fluorescence Microscope, Microscope
Form: Liquid
Label or Dye: GFP (EmGFP)
Product Size: 100 µL
Sub-Cellular Localization: Mitochondria
Technique: Fluorescence Intensity, In Vivo Imaging
Shipping Condition: Wet Ice

Contents & storage

Store at 2-6° C, protected from light. Do Not Freeze.

Documents

Manuals & protocols

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