CellLight® MAP4-RFP, BacMam 2.0 - FAQs

CellLight® MAP4-RFP, BacMam 2.0 - FAQs

View additional product information for CellLight® MAP4-RFP, BacMam 2.0 - FAQs (C10599)

1 product FAQs found

I keep getting low transduction efficiency when using CellLight labeling reagents on my neurons. What can I do to improve the efficiency?

Neurons are more difficult to transduce than many other cells. The main way to improve transduction is to label with a higher number of particles per cell. For primary neurons, it can also help to transduce them at the time of plating rather than on established cultures. There can also be a slower onset of expression in neurons and peak expression often occurs on day 2-3 rather than 16 hours after transduction.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.