Seeing the trees in the forest: selective electroporation of adipocytes within adipose tissue.
AuthorsGranneman JG, Li P, Lu Y, Tilak J
JournalAm J Physiol Endocrinol Metab
PubMed ID15126244
'Electroporation has been recently adapted for the transfer of macromolecules into cells of tissues in vivo. Although mature adipocytes constitute <20% of cells residing in adipose tissue, we hypothesized that fat cells might be susceptible to selective electrotransfer of plasmid DNA owing to their large size relative to other cells ... More
Lectin mapping reveals stage-specific display of surface carbohydrates in in vitro and haemolymph-derived cells of the entomopathogenic fungus Beauveria bassiana.
AuthorsWanchoo A, Lewis MW, Keyhani NO,
JournalMicrobiology
PubMed ID19608611
'The entomopathogenic fungus Beauveria bassiana and its insect host target represent a model system with which to examine host-pathogen interactions. Carbohydrate epitopes on the surfaces of fungal cells play diverse roles in processes that include adhesion, non-self recognition and immune invasion with respect to invertebrate hosts. B. bassiana produces a ... More
In vitro growth and analysis of Candida biofilms.
AuthorsChandra J, Mukherjee PK, Ghannoum MA,
JournalNat Protoc
PubMed ID19180075
'Evaluation of fungal biofilm formation can be performed using several techniques. In this protocol, we describe methods used to form Candida biofilms on three different medical device substrates (denture strips, catheter disks and contact lenses) to quantify them and to evaluate their architecture and drug susceptibility. Biofilm formation involves adhesion ... More
Phosphorylation of tumor necrosis factor receptor CD120a (p55) by p42(mapk/erk2) induces changes in its subcellular localization.
AuthorsCottin V, Van Linden A, Riches DW
JournalJ Biol Chem
PubMed ID10551865
'The interaction of tumor necrosis factor-alpha (TNFalpha) with its receptor sets in motion downstream signaling events including the activation of members of the mitogen-activated protein kinase (MAPK) family. In this study, we show that p42(mapk/erk2) phosphorylates sequences present within the cytoplasmic domain of CD120a (p55). By using a GST-CD120a-(207-425) fusion ... More
Dynamic microcompartmentation in synthetic cells.
AuthorsLong MS, Jones CD, Helfrich MR, Mangeney-Slavin LK, Keating CD
JournalProc Natl Acad Sci U S A
PubMed ID15788532
'An experimental model for cytoplasmic organization is presented. We demonstrate dynamic control over protein distribution within synthetic cells comprising a lipid bilayer membrane surrounding an aqueous polymer solution. This polymer solution generally exists as two immiscible aqueous phases. Protein partitioning between these phases leads to microcompartmentation, or heterogeneous protein distribution ... More
G proteins mediate changes in cell shape by stabilizing the axis of polarity.
AuthorsNern A, Arkowitz RA
JournalMol Cell
PubMed ID10882121
Upon exposure to mating pheromone, yeast cells change their form to pear-shaped shmoos. We looked at pheromone-dependent cell shape changes in mutants that are unable to orient growth during mating and unable to choose a bud site. In these double mutants, cell surface growth, secretion sites, cytoskeleton, and pheromone receptors ... More
Multiparameter assessments to determine the effects of sugars and antimicrobials on a polymicrobial oral biofilm.
AuthorsYang Y, Sreenivasan PK, Subramanyam R, Cummins D,
JournalAppl Environ Microbiol
PubMed ID17021225
Clinical studies indicate relationships between dental plaque, a naturally formed biofilm, and oral diseases. The crucial role of nonmicrobial biofilm constituents in maintaining biofilm structure and biofilm-specific attributes, such as resistance to shear and viscoelasticity, is increasingly recognized. Concurrent analyses of the diverse nonmicrobial biofilm components for multiparameter assessments formed ... More
Fluorescence visualization of branchial collagen columns embraced by pillar cells.
AuthorsKudo H, Kato A, Hirose S
JournalJ Histochem Cytochem
PubMed ID16957162
A collagen column is a structure of the extracellular matrix that helps to maintain the flatness and width of gill lamella. Collagen columns are unique in that they are enfolded by plasma membrane of pillar cells that form two-dimensional vascular networks between parallel sheets of respiratory epithelia. Despite their unique ... More
Helical disposition of proteins and lipopolysaccharide in the outer membrane of Escherichia coli.
AuthorsGhosh AS, Young KD
JournalJ Bacteriol
PubMed ID15743937
In bacteria, several physiological processes once thought to be the products of uniformly dispersed reactions are now known to be highly asymmetric, with some exhibiting interesting geometric localizations. In particular, the cell envelope of Escherichia coli displays a form of subcellular differentiation in which peptidoglycan and outer membrane proteins at ... More
A fluorescence affinity hollow fiber sensor for continuous transdermal glucose monitoring.
AuthorsBallerstadt R, Schultz JS
JournalAnal Chem
PubMed ID10994982
A novel concept of a fluorescence affinity hollow fiber sensor for transdermal glucose monitoring is demonstrated. The glucose-sensing principle is based on the competitive reversible binding of a mobile fluorophore-labeled Concanavalin A (Con A) to immobile pendant glucose moites inside of intensely colored Sephadex beads. The highly porous beads (molecular ... More
Antifungal activity of amiodarone is mediated by disruption of calcium homeostasis.
AuthorsGupta SS, Ton VK, Beaudry V, Rulli S, Cunningham K, Rao R
JournalJ Biol Chem
PubMed ID12754197
The antiarrhythmic drug amiodarone was recently demonstrated to have novel broad range fungicidal activity. We provide evidence that amiodarone toxicity is mediated by disruption of Ca2+ homeostasis in Saccharomyces cerevisiae. In mutants lacking calcineurin and various Ca2+ transporters, including pumps (Pmr1 and Pmc1), channels (Cch1/Mid1 and Yvc1), and exchangers (Vcx1), ... More
S-nitrosoprotein formation and localization in endothelial cells.
AuthorsYang Y, Loscalzo J
JournalProc Natl Acad Sci U S A
PubMed ID15618409
Protein S-nitrosation represents a recently described form of post-translational modification that is rapid and reversible. However, the analysis of protein S-nitrosation in situ has been difficult because of the absence of specific probes and the instability of cellular protein S-nitrosothiols. We developed a rapid and specific method for detecting endothelial ... More
Comparison of biofilms formed by Candida albicans and Candida parapsilosis on bioprosthetic surfaces.
AuthorsKuhn DM, Chandra J, Mukherjee PK, Ghannoum MA
JournalInfect Immun
PubMed ID11796623
Little is known about fungal biofilms, which may cause infection and antibiotic resistance. In this study, biofilm formation by different Candida species, particularly Candida albicans and C. parapsilosis, was evaluated by using a clinically relevant model of Candida biofilm on medical devices. Candida biofilms were allowed to form on silicone ... More
Antifungal potential of Sideroxylon obtusifolium and Syzygium cumini and their mode of action against Candida albicans.
AuthorsPereira JV, Freires IA, Castilho AR, da Cunha MG, Alves Hda S, Rosalen PL
JournalPharm Biol
PubMed ID26987037
'Context The emergence of resistant pathogens and toxicity of antifungals have encouraged an active search for novel candidates to manage Candida biofilms. Objective In this study, the little known species Sideroxylon obtusifolium T.D. Penn (Sapotacea) and Syzygium cumini (L.) Skeels (Myrtaceae), from the Caatinga biome in Brazil were chemically characterized ... More
Nanostructured Coating for Biomaterial Lubrication through Biomacromolecular Recruitment.
Authors
JournalACS Appl Mater Interfaces
PubMed ID32347093
MS4A15 drives ferroptosis resistance through calcium-restricted lipid remodeling.
Authors
JournalCell Death Differ
PubMed ID34663908
AQP8 is a crucial H2O2 transporter in insulin-producing RINm5F cells.
Authors
JournalRedox Biol
PubMed ID33892285
A high-resolution imaging approach to investigate chromatin architecture in complex tissues.
Authors
JournalCell
PubMed ID26406379
Spatial control of Cdc42 signalling by a GM130-RasGRF complex regulates polarity and tumorigenesis.
Authors
JournalNat Commun
PubMed ID25208761
The Galapagos Chip Platform for High-Throughput Screening of Cell Adhesive Chemical Micropatterns.
Authors
JournalSmall
PubMed ID34985808
Integrating deep learning and unbiased automated high-content screening to identify complex disease signatures in human fibroblasts.
Authors
JournalNat Commun
PubMed ID35338121
Natural product fragment combination to performance-diverse pseudo-natural products.
Authors
JournalNat Commun
PubMed ID33767198
Control of osteocyte dendrite formation by Sp7 and its target gene osteocrin.
Authors
JournalNat Commun
PubMed ID34725346
Development of the Theta Comparative Cell Scoring Method to Quantify Diverse Phenotypic Responses Between Distinct Cell Types.
AuthorsWarchal SJ, Dawson JC, Carragher NO
JournalAssay Drug Dev Technol
PubMed ID27552144
In this article, we have developed novel data visualization tools and a Theta comparative cell scoring (TCCS) method, which supports high-throughput in vitro pharmacogenomic studies across diverse cellular phenotypes measured by multiparametric high-content analysis. The TCCS method provides a univariate descriptor of divergent compound-induced phenotypic responses between distinct cell types, ... More
Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes.
AuthorsBray MA, Singh S, Han H, Davis CT, Borgeson B, Hartland C, Kost-Alimova M, Gustafsdottir SM, Gibson CC, Carpenter AE
JournalNat Protoc
PubMed ID27560178
In morphological profiling, quantitative data are extracted from microscopy images of cells to identify biologically relevant similarities and differences among samples based on these profiles. This protocol describes the design and execution of experiments using Cell Painting, which is a morphological profiling assay that multiplexes six fluorescent dyes, imaged in ... More
Olfactory receptors are expressed in pancreatic ß-cells and promote glucose-stimulated insulin secretion.
AuthorsMunakata Y, Yamada T, Imai J, Takahashi K, Tsukita S, Shirai Y, Kodama S, Asai Y, Sugisawa T, Chiba Y, Kaneko K, Uno K, Sawada S, Hatakeyama H, Kanzaki M, Miyazaki JI, Oka Y, Katagiri H
JournalSci Rep
PubMed ID29367680
Olfactory receptors (ORs) mediate olfactory chemo-sensation in OR neurons. Herein, we have demonstrated that the OR chemo-sensing machinery functions in pancreatic ß-cells and modulates insulin secretion. First, we found several OR isoforms, including OLFR15 and OLFR821, to be expressed in pancreatic islets and a ß-cell line, MIN6. Immunostaining revealed OLFR15 ... More
Multinuclear Ru(ii) and Ir(iii) decorated tetraphenylporphyrins as efficient PDT agents.
AuthorsCabrera-González J, Soriano J, Conway-Kenny R, Wang J, Lu Y, Zhao J, Nogués C, Draper SM
JournalBiomater Sci
PubMed ID31187805
Two novel porphyrin-core systems were prepared by Sonogashira cross-coupling of the terminal alkyne groups of meso-tetra(4-ethynylphenyl)porphyrin-Zn(ii) (P-1) with halogenated Ru(ii)- or Ir(iii)-phenanthroline complexes. The resulting compounds (P-Ru and P-Ir) were spectroscopically characterised and their photophysical properties were investigated (? ... More
Dried Biofilms of Desert Strains of
AuthorsBilli D, Staibano C, Verseux C, Fagliarone C, Mosca C, Baqué M, Rabbow E, Rettberg P
JournalAstrobiology
PubMed ID30741568
Dried biofilms and dried multilayered planktonic counterparts obtained from three desert strains of
Direct Detection of Heterotrophic Diazotrophs Associated with Planktonic Aggregates.
AuthorsGeisler E, Bogler A, Rahav E, Bar-Zeev E
JournalSci Rep
PubMed ID31243322
N
Biofilm inhibiting properties of compounds from the leaves of Warburgia ugandensis Sprague subsp ugandensis against Candida and staphylococcal biofilms.
AuthorsKipanga PN, Liu M, Panda SK, Mai AH, Veryser C, Van Puyvelde L, De Borggraeve WM, Van Dijck P, Matasyoh J, Luyten W
JournalJ Ethnopharmacol
PubMed ID31676401
Warburgia ugandensis Sprague subspecies ugandensis is a plant widely distributed in Eastern, Central and Southern Africa. In humans, it is used to treat respiratory infections, tooth aches, malaria, skin infections, venereal diseases, diarrhea, fevers and aches. ... More
A novel Golgi mannosidase inhibitor: Molecular design, synthesis, enzyme inhibition, and inhibition of spheroid formation.
AuthorsKoyama R, Kano Y, Kikushima K, Mizutani A, Soeda Y, Miura K, Hirano T, Nishio T, Hakamata W
JournalBioorg Med Chem
PubMed ID32291147
Effective chemotherapy for solid cancers is challenging due to a limitation in permeation that prevents anticancer drugs from reaching the center of the tumor, therefore unable to limit cancer cell growth. To circumvent this issue, we planned to apply the drugs directly at the center by first collapsing the outer ... More