Invitrogen™

ChromaTide™ Alexa Fluor™ 488-5-UTP

Catalog number: C11403
Have Questions?
Invitrogen™

ChromaTide™ Alexa Fluor™ 488-5-UTP

Catalog number: C11403
Have Questions?
Catalog Number
C11403
Unit Size
25 µL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C1140325 µL
-
Product Overview
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Molecular Probes™ ChromaTide™ dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide™ dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis.

ChromaTide™ Labeled Nucleotides Specifications:
• Ex/Em of dye: Alexa Fluor™ 488-5-UTP (490/520 nm)
• Length of alkynylamino linker: 5 atoms


Methods for Incorporating ChromaTide™ Nucleotides Into Probes
• Nick translation
• Random primer labeling
• End-labeling with terminal deoxynucleotidyl transferase
• Reverse transcription
• PCR amplification


See Methods for Enzymatic Incorporation of ChromaTide™ dUTPs for specific guidelines for each of these methods.

Alexa Fluor™ and BODIPY™ Fluorescent Dyes Make Excellent Probes
Probes made with labeled nucleotides can be used for multicolor techniques such as in situ hybridization and hybridization to arrays. Our proprietary BODIPY™ and Alexa Fluor™ dye conjugates are exceptionally bright, photostable, and essentially pH insensitive. The narrow emission profile of the BODIPY™ dyes helps ensure minimal spectral overlap. The Alexa Fluor™ dyes are highly water soluble, as are DNA probes made from them, making them the labels of choice for fluorescence in situ hybridization.

Long Linkers Improve Performance
The ChromaTide™ dUTP and UTP nucleotides are modified at the C-5 position of uridine via a unique alkynylamino linker, which provides a spacer between the nucleotide and the dye to reduce interactions between them. The number in the product name, e.g., the “12” in fluorescein-12-dUTP, indicates the net length of the spacer, in atoms. These spacers result in brighter conjugates and increased hapten accessibility for secondary detection reagents.

For complete listing of our ChromaTide™ Reagents: Molecular Probes ChromaTide™ and aha labeled nucleotides—Table 8.5.
For additional information on these labeling reagents, read Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes™ Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Labeling Method
Direct Labeling
Shipping Condition
Dry Ice
Concentration
1 mM
Label or Dye
Alexa Fluor™ 488
Quantity
25 μL
Product Line
Alexa Fluor™, ChromaTide™

Contents & Storage

Store in freezer (-5 to -30°C) and protect from light.

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