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CyQUANT™ LDH Cytotoxicity Assay, fluorescence, 1000 assays - FAQs

CyQUANT™ LDH Cytotoxicity Assay, fluorescence, 1000 assays - FAQs

View additional product information for CyQUANT™ LDH Cytotoxicity Assays - FAQs (C20301, C20300, C20303, C20302)

4 product FAQs found

How many assays can I perform using the CyQUANT LDH Cytotoxicity Assay - Fluorescence Kit (Cat. No. C20303)?

The CyQUANT LDH Cytotoxicity Assay - Fluorescence Kit (Cat. No. C20303) contains sufficient reagents to perform 1,000 assays in 96-well plates.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

In the CyQUANT LDH Cytotoxicity Fluorescence assay, my treatment resulted in an LDH release signal that was lower than the Spontaneous LDH Release Control signal. What could cause this?

Here are potential causes:
- Fluorescence may be quenched and/or LDH activity may be inhibited by various chemical compounds.
- The LDH may be precipitated/denatured.
- LDH can also be digested by any proteases liberated in the extracellular medium, examine the samples for protease activity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I did not have a Spontaneous LDH Release Control in the CyQUANT LDH Cytotoxicity Fluorescence assay? Is this critical?

Yes. The Spontaneous LDH Release Control provides the lower limit of natural LDH release for untreated samples (healthy cells). Without this control, you cannot determine whether any treatment resulted in any change in release, which relates to levels of cytotoxicity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I refrigerate or freeze the collected cell culture media for analysis later using the CyQUANT LDH Cytotoxicity Assay - Fluorescence Kit?

We do not recommend doing this. LDH is an enzyme that will lose activity in diluted form when stored over time, refrigeration would not limit this loss of activity and freezing would cause a greater loss of activity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.