Invitrogen™

CellTracker™ Fluorescent Probes

Catalog number: C2111
Invitrogen™

CellTracker™ Fluorescent Probes

Catalog number: C2111
Cell movement and location studies require specialized probes that are nontoxic to living cells and well retained, allowing for multigenerational tracking. The CellTracker fluorescent probes are available in a range of fluorescent colors to match instrument lasers and filters, and to accommodate co-staining with antibodies or other cell analysis probes. These dyes are excellent tools for monitoring cell movement, location, proliferation, migration, chemotaxis, and invasion.
 
Catalog Number
C2111
Unit Size
5 mg
Description
CellTracker™ Blue CMHC Dye, 5 mg
Color
Blue
Form
Dry Powder
Price (USD)
Availability
-
Full specifications
Quantity
ColorBlue
Quantity5 mg
DescriptionCellTracker™ Blue CMHC Dye, 5 mg
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Unit Size5 mg
Catalog Number
Unit Size
5 mg
Description
CellTracker™ Blue CMF2HC Dye, 5 mg
Color
Blue
Form
Dry Powder
Price (USD)
Availability
-
Full specifications
Quantity
ColorBlue
Excitation Wavelength Range371/464 nm
Quantity5 mg
DescriptionCellTracker™ Blue CMF2HC Dye, 5 mg
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Unit Size5 mg
Catalog Number
Unit Size
5 mg
Description
CellTracker™ Blue CMAC Dye, 5 mg
Color
Blue
Form
Dry Powder
Price (USD)
Availability
-
Full specifications
Quantity
ColorBlue
Excitation Wavelength Range466 nm
Quantity5 mg
DescriptionCellTracker™ Blue CMAC Dye, 5 mg
Emission353 nm
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Molecular Weight (g/mol)209.63
Unit Size5 mg
Catalog Number
Unit Size
5 mg
Description
CellTracker™ Green BODIPY™ Dye, 5 mg
Color
Green
Form
Dry Powder
Price (USD)
Availability
-
Full specifications
Quantity
ColorGreen
Dye TypeBODIPY Dyes
Quantity5 mg
DescriptionCellTracker™ Green BODIPY™ Dye, 5 mg
FormDry Powder
Product LineBODIPY™, CellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Unit Size5 mg
Catalog Number
Unit Size
20 x 50 µg
Description
CellTracker™ Green CMFDA Dye, 20 x 50 μg
Color
Green
Form
Dry Powder
Price (USD)
Availability
-
Full specifications
Quantity
ColorGreen
Excitation Wavelength Range492/517 nm
Dye TypeFluorescent dye
For Use With (Application)Fluroescence microscopy, cells in suspension, adherent cells
Quantity20 x 50 μg
Detection MethodFluroescent probes
DescriptionCellTracker™ Green CMFDA Dye, 20 x 50 μg
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Molecular Weight (g/mol)464.86
Unit Size20 x 50 µg
Catalog Number
Unit Size
1 mg
Description
CellTracker™ Green CMFDA Dye, 1 mg
Color
Green
Form
Dry Powder
Price (USD)
Availability
-
Full specifications
Quantity
ColorGreen
Quantity1 mg
DescriptionCellTracker™ Green CMFDA Dye, 1 mg
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Unit Size1 mg
Catalog Number
Unit Size
20 x 50 µg
Description
CellTracker™ Orange CMRA Dye, 20 x 50 μg
Color
Orange
Form
Dry Powder
Price (USD)
Availability
-
Full specifications
Quantity
ColorOrange
Excitation Wavelength Range548/576 nm
Quantity20 x 50 μg
DescriptionCellTracker™ Orange CMRA Dye, 20 x 50 μg
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Unit Size20 x 50 µg
Catalog Number
Unit Size
1 mg
Description
CellTracker™ Orange CMTMR Dye, 1 mg
Color
Orange
Form
Dry Powder
Price (USD)
Availability
-
Full specifications
Quantity
ColorOrange
Quantity1 mg
DescriptionCellTracker™ Orange CMTMR Dye, 1 mg
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Unit Size1 mg
Catalog Number
Unit Size
20 x 15 µg
Description
CellTracker™ Deep Red Dye, 20 x 15 μg
Color
Deep Red
Form
Dry Powder
Price (USD)
Availability
-
Full specifications
Quantity
ColorDeep Red
For Use With (Application)Imaging
Quantity20 x 15 μg
Detection MethodFluorescence
DescriptionCellTracker™ Deep Red Dye, 20 x 15 μg
Emission630/660 nm
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds
Label TypeOther Labels or Dyes
Product TypeDye
Unit Size20 x 15 µg
Catalog Number
Unit Size
20 x 50 µg
Description
CellTracker™ Red CMTPX Dye, 20 x 50 μg
Color
Red
Form
Dry Powder
Price (USD)
Availability
-
Full specifications
Quantity
ColorRed
Excitation Wavelength Range577/602 nm
Quantity20 x 50 μg
DescriptionCellTracker™ Red CMTPX Dye, 20 x 50 μg
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Unit Size20 x 50 µg
Showing 10 of 11Show more
Catalog NumberSpecificationsUnit SizeDescriptionColorFormPrice (USD)AvailabilityQuantity
C2111Full specifications
5 mgCellTracker™ Blue CMHC Dye, 5 mgBlueDry Powder
-
ColorBlue
Quantity5 mg
DescriptionCellTracker™ Blue CMHC Dye, 5 mg
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Unit Size5 mg
C12881Full specifications
5 mgCellTracker™ Blue CMF2HC Dye, 5 mgBlueDry Powder
-
ColorBlue
Excitation Wavelength Range371/464 nm
Quantity5 mg
DescriptionCellTracker™ Blue CMF2HC Dye, 5 mg
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Unit Size5 mg
C2110Full specifications
5 mgCellTracker™ Blue CMAC Dye, 5 mgBlueDry Powder
-
ColorBlue
Excitation Wavelength Range466 nm
Quantity5 mg
DescriptionCellTracker™ Blue CMAC Dye, 5 mg
Emission353 nm
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Molecular Weight (g/mol)209.63
Unit Size5 mg
C2102Full specifications
5 mgCellTracker™ Green BODIPY™ Dye, 5 mgGreenDry Powder
-
ColorGreen
Dye TypeBODIPY Dyes
Quantity5 mg
DescriptionCellTracker™ Green BODIPY™ Dye, 5 mg
FormDry Powder
Product LineBODIPY™, CellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Unit Size5 mg
C7025Full specifications
20 x 50 µgCellTracker™ Green CMFDA Dye, 20 x 50 μgGreenDry Powder
-
ColorGreen
Excitation Wavelength Range492/517 nm
Dye TypeFluorescent dye
For Use With (Application)Fluroescence microscopy, cells in suspension, adherent cells
Quantity20 x 50 μg
Detection MethodFluroescent probes
DescriptionCellTracker™ Green CMFDA Dye, 20 x 50 μg
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Molecular Weight (g/mol)464.86
Unit Size20 x 50 µg
C2925Full specifications
1 mgCellTracker™ Green CMFDA Dye, 1 mgGreenDry Powder
-
ColorGreen
Quantity1 mg
DescriptionCellTracker™ Green CMFDA Dye, 1 mg
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Unit Size1 mg
C34551Full specifications
20 x 50 µgCellTracker™ Orange CMRA Dye, 20 x 50 μgOrangeDry Powder
-
ColorOrange
Excitation Wavelength Range548/576 nm
Quantity20 x 50 μg
DescriptionCellTracker™ Orange CMRA Dye, 20 x 50 μg
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Unit Size20 x 50 µg
C2927Full specifications
1 mgCellTracker™ Orange CMTMR Dye, 1 mgOrangeDry Powder
-
ColorOrange
Quantity1 mg
DescriptionCellTracker™ Orange CMTMR Dye, 1 mg
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Unit Size1 mg
C34565Full specifications
20 x 15 µgCellTracker™ Deep Red Dye, 20 x 15 μgDeep RedDry Powder
-
ColorDeep Red
For Use With (Application)Imaging
Quantity20 x 15 μg
Detection MethodFluorescence
DescriptionCellTracker™ Deep Red Dye, 20 x 15 μg
Emission630/660 nm
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds
Label TypeOther Labels or Dyes
Product TypeDye
Unit Size20 x 15 µg
C34552Full specifications
20 x 50 µgCellTracker™ Red CMTPX Dye, 20 x 50 μgRedDry Powder
-
ColorRed
Excitation Wavelength Range577/602 nm
Quantity20 x 50 μg
DescriptionCellTracker™ Red CMTPX Dye, 20 x 50 μg
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCytoplasm
Unit Size20 x 50 µg
Showing 10 of 11Show more

Features of the CellTracker dyes include:
Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells
Excellent retention—fluorescent signal retention of >72 hours (typically three to six generations)
Ideal tracking dyes—monitor cell movement, location, proliferation, migration, chemotaxis, and invasion
Low cytotoxicity—does not affect viability or proliferation
Cell Versatility– works with cells in suspension and adherent cells

The CellTracker fluorescent probes have been designed to freely pass through cell membranes; however, once inside the cell are transformed into cell-impermeant reaction products. The CellTracker fluorescence probes (except for CellTracker Deep Red) contain a chloromethyl or bromomethyl group that reacts with thiol groups, utilizing a glutathione S-transferase–mediated reaction. In most cells, glutathione levels are high (up to 10 mM) and glutathione transferase is ubiquitous. CellTracker Deep Red probe contains a succinimidyl ester reactive group, which reacts with amine groups present on proteins.

After conversion to impermeant versions, the CellTracker fluorescent probes are well retained in living cells through several generations. The probes are transferred to daughter cells, but are not transferred to adjacent cells in a population. Cells loaded with the CellTracker fluorescent probes display fluorescence for at least 72 hours and exhibit ideal tracking dye properties—they are stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, several CellTracker fluorescent probes with various excitation and emission spectra are available allowing for multiplexing in fluorescence imaging. The CellTracker dyes are retained with fixation and permeabilization, enabling their use with antibodies for fluorescence immunostaining applications.

Spectral characteristics of the fluorescent CellTracker probes:
• CellTracker Blue CMAC—353/466 nm
• CellTracker Blue CMF2HC—371/464 nm
• CellTracker Blue CMHC—372/470 nm
• CellTracker Violet BMQC—415/516 nm
 CellTracker Green CMFDA—492/517 nm
• CellTracker Green BODIPY™—522/529 nm
 CellTracker Orange CMTMR—541/565 nm
• CellTracker Orange CMR—548/576 nm
• CellTracker Red CMTPX—577/602 nm
• CellTracker Deep Red—630/660 nm

Fluorescent CellTracker reagents include the blue-fluorescent chloromethyl derivatives of amino-, hydroxy-, and difluorohydroxycoumarin (CMAC, CMHC and CMF2HC), the green-fluorescent chloromethyl derivatives of fluorescein diacetate (CMFDA) and a BODIPY™ dye, the orange-fluorescent CMTMR and CMRA, and the red-fluorescent CMTPX. CellTracker Blue CMAC, CMHC, and CMF2HC, CellTracker Violet, the violet-fluorescent bromomethyl derivative of coumarin (BMQC), CellTracker Green BODIPY™, CellTracker Orange CMTMR, and CellTracker Red CMTPX do not require enzymatic cleavage to activate their fluorescence, whereas the green CMFDA and orange CMRA do require enzymatic cleavage. The impermeable reaction products of the chloromethyl or bromomethyl coumarins have excellent retention, strong fluorescence, and relatively uniform cytoplasmic staining, making these derivatives potentially useful for correcting motion artifacts in imaging. CMFDA is colorless and non-fluorescent until cytosolic esterases cleave off the acetates, releasing a brightly fluorescent product.

In addition to bright fluorescence and excellent retention, the CellTracker fluorescent probes do not contribute to cytotoxicity.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Store in freezer -5°C to -30°C and protect from light.

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