CellTracker Green CMFDA (5-chloromethylfluorescein diacetate) is a fluorescent dye well suited for monitoring cell movement or location. After loading into cells, the dye is well retained, allowing for multigenerational tracking of cellular movements. The green excitation/emission spectra are ideal for multiplexing with red fluorescent dyes and proteins.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Green excitation/emission spectra (492/517 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation

CellTracker Green CMFDA fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into a cell-impermeant, fluorescent product. CellTracker Green fluorescent dyes are retained in living cells through several generations. The dyes are transferred to daughter cells but not adjacent cells in a population. CellTracker Green CMFDA dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker Green CMFDA dye are well separated from RFP (red fluorescent protein) spectra allowing for multiplexing.

See User Manual for solubility instructions.