Casein, Fluorescein conjugate

Catalog number: C2990

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Protein Assays and Analysis

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Description

Fluorescein casein can be used to assay protease activity. In this assay, unhydrolyzed fluorescein casein must be precipitated with trichloroacetic acid, separated by centrifugation, transferred for measurement and then pH-adjusted for fluorescein signal enhancement. The EnzChek® Protease Assay Kits (E6638, E6639) also use a fluorescently-labeled casein derivative, but unlike fluorescein casein, these substrates do not require any precipitation or separation steps for detection.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Substrate: Casein
Product Size: 25 mg
Substrate Type: Protease Substrate
Detection Method: Fluorescent
Substrate Properties: Protein-Based Substrate
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

Documents

Manuals & protocols