CellTrace™ Blue Cell Proliferation Kit, for flow cytometry, 180 Assays

CellTrace™ Blue Cell Proliferation Kit, for flow cytometry, 180 Assays - FAQs

View additional product information for CellTrace™ Blue Cell Proliferation Kit, for flow cytometry - FAQs (C34574, C34568)

3 product FAQs found

I would like to label two cell populations with two different CellTrace reagents and then co-culture these cells. Will the CellTrace reagent leave the cells to stain other cells?

We have not tested the use of the CellTrace reagents for co-culture applications. In theory, this may work, but you would have to test this on your cells of interest.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

With the CellTrace cell proliferation kits, for flow cytomtery, I am getting only one broad peak on my histogram instead of multiple peaks. What is causing this?

A single broad peak is usually caused by using too high a concentration of dye and/or too long an incubation time.

Find additional tips, troubleshooting help, and resources within our Flow Cytometry Support Center.

What cellular components do the CellTrace Proliferation dyes label?

The dyes provided in the CellTrace Proliferation kits are amine-reactive dyes that are considered general cytoplasmic stains. They may bind to various membrane proteins, organelles as well as components in the cytoplasm. They are not known to localize in any specific organelles.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.