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One Shot® TOP10 Chemically Competent E. coli

Catalog number: C404003

Invitrogen™  Related applications: Transformation

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Description

One Shot® TOP10 Chemically Competent E. coli are provided at a transformation efficiency of 1 x 109 cfu/µg plasmid DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids and are the same competent cells that come with many of our cloning kits.

Features of One Shot® TOP10 cells include:

• Maximization of cloning efficiency in a single-tube format
• Enhanced genomic DNA cloning capabilities

Easy-to-use One Shot® format
The single-tube, single-use format allows for all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping to save time and prevent contamination.

Versatile cloning capabilities
One Shot® TOP10 E. coli cells are similar to the DH10B™ strain, and offer the following features:

• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Genotype: F- mcrA Δ( mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ( araleu)7697 galU galK rpsL (StrR) endA1 nupG

Find the strain and format that you need
We also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require high-throughput transformation, choose from our collection of MultiShot™ formatted comp cells.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Chemically Competent
Species: E. coli
Format: One Shot
Transformation Efficiency: > 1x10^9
Bacterial or Yeast Strain: TOP10
Blue-White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
F' Episome (to make ssDNA): Lacks F' Episome
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Improves Plasmid Quality: Yes
Plasmid Type: High Copy Plasmid
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Size: 20 reactions
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
T1 Phage - Resistant (tonA): No
Shipping Condition: Dry Ice

Contents & storage

Contains:
• One Shot® TOP10 Chemically Competent E. coli: 21 vials, 50 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

Documents

Manuals & protocols

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