Invitrogen™

MultiShot™ FlexPlate TOP10 Competent Cells

Catalog number: C4081201
Have Questions?
Invitrogen™

MultiShot™ FlexPlate TOP10 Competent Cells

Catalog number: C4081201
Have Questions?
Catalog Number
C4081201
also known as C40812-01
Unit Size
1 x 96 well plate (20 µl/well)
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C4081201
also known as C40812-01
1 x 96 well plate (20 µl/well)
-
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MultiShot FlexPlate TOP10 chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments to allow for flexibility in transformation throughput.

Versatile TOP10 cloning capabilities
MultiShot FlexPlate TOP10 Competent Cells are the laboratory workhorse for chemical transformations as they are compatible with a broad spectrum of DNA types for transformation. The TOP10 strain is ideal for high-efficiency cloning and plasmid propagation, achieving transformation efficiency of >1 x 108 cfu/μg with control plasmid DNA in FlexPlate format. It allows stable replication of high-copy number plasmids and is the same competent cell strain that come with many of our cloning kits.

TOP10 E. coli cells are genetically similar to the DH10B strain, but has been reported to be more resilient to stress conditions (osmotic shock and acidic pH stress). These cells carry mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr), allowing the cloning of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. In addition, recA1 and endA1 mutations increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

MultiShot FlexPlate TOP10 Competent Cells offer:
• Transformation efficiencies of >1 x 108 cfu/μg
hsdR for efficient transformation of unmethylated DNA from PCR amplifications
mcrA for efficient transformation of methylated DNA from genomic preparations
lacZΔM15 for blue/white color screening of recombinant clones on plates containing either X-Gal or Bluo-Gal
endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
recA1 for reduced occurrence of non-specific recombination in cloned DNA

Note: TOP10 cells do not require IPTG to induce expression from the lac promoter.

Increase transformation productivity with MultiShot FlexPlate format
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler.

Key benefits of the MultiShot FlexPlate format
Increased throughput—compatible with automated liquid handling platforms
Flexible—transform in increments of 8 wells to entire 96-well plate
Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype
FmcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK λ–rpsL(StrR) endA1 nupG

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet specific transformation needs. For high-throughput transformation, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options. Bacterial growth media is also available in various formats.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Chemically Competent Cells
Contains F' Episome
No
Improves Plasmid Quality
Yes (endA1)
Cloning Methylated DNA
Yes (mcrA)
Antibiotic Resistance Bacterial
Yes (Streptomycin)
Cloning Unstable DNA
No
Blue/White Screening
Yes (lacZΔM15)
High-throughput Compatibility
High
Plasmid
High Copy Plasmid
Preparing Unmethylated DNA
No
Reduces Recombination
Yes (recA1)
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
No
Species
E. coli (K12)
Format
96-well Plate
Product Line
Multishot™
Quantity
1 x 96-well Plate

Contents & Storage

• MultiShot FlexPlate TOP10 Competent Cells (1 x 96-well plate at 20 μL/well)
Store at –80°C.

• pUC19 DNA (50 μL at 10 pg/μL)
Store at –20°C.

• S.O.C. Medium (2 x 10 mL)
Store at 4°C or room temperature.

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