MultiShot™ StripWell TOP10 Chemically Competent E. coli

Catalog number: C409601

Invitrogen™  Related applications: Transformation

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Description

MultiShot™ StripWell TOP10 Chemically Competent E. coli provide transformation efficiencies of >1 x 108 cfu/µg with control plasmid DNA. MultiShot™ StripWell TOP10 Chemically Competent cells are packaged in a 96-stripwell plate (50 µL aliquots) to simplify medium- and high-throughput bacterial transformation. Features of these cells:

• Flexible—designed for medium- to high-throughput cloning
• Compatible with genomic DNA—the mcrA mutation supports efficient transfection of methylated DNA
• Convenient —the lacZΔM15 allele permits rapid blue/white screening of transfectants on plates containing X-Gal or Bluo-Gal
• Efficient—the endA1 mutation reduces endonuclease activity, improving plasmid DNA yields
• Reliable—the recA1 mutation reduces unwanted recombination

Flexible use for medium- to high-throughput transformations
MultiShot™ StripWell TOP10 Chemically Competent cells are genetically similar to the reliable DH10B™ strain. After addition of DNA, MultiShot™ StripWell TOP10 Chemically Competent cells can be transformed by heat-shock at 42°C using a water bath. Using our TOPO® cloning protocol and control vector, transformation efficiencies of >1 x 108 cfu/µg plasmid DNA, and yields of 100–300 colonies per agar plate can be achieved.

Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) endA1 nupG

Find the strain and format that you need
We also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot™ formatted comp cells or contact us for custom formatting options.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Chemically Competent
Species: E. coli
Format: MultiShot StripWell
Transformation Efficiency: > 1x10^8
Bacterial or Yeast Strain: TOP10
Blue-White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
F' Episome (to make ssDNA): Lacks F' Episome
High Throughput Compatibility: High Throughput-Compatible
Improves Plasmid Quality: Yes
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Size: 1 plate
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
T1 Phage - Resistant (tonA): No

Contents & storage

Contains:
• MultiShot™ StripWell TOP10 Chemically Competent E. coli: One 96-stripwell plate, 50 µl per well
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 2 bottles, 15 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

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