One Shot® BL21(DE3)pLysS Chemically Competent E. coli

Catalog number: C606010

Invitrogen™  Related applications: Protein Expression

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Description

BL21(DE3)pLysS cells are ideal for use with T7 promoter-based expression systems (e.g., pRSET, pCR™T7, and pET). BL21(DE3)pLysS E. coli carry both the DE3 lysogen and the plasmid pLysS. pLysS constitutively expresses low levels of T7 lysozyme, which reduces basal expression of recombinant genes by inhibiting basal levels of T7 RNA polymerase.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Chemically Competent
Species: E. coli
Format: One Shot
Blue-White Screening: No
Bacterial or Yeast Strain: BL21(DE3), BL21(DE3)pLysS
Cloning Methylated DNA: No
F' Episome (to make ssDNA): Lacks F' Episome
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Improves Plasmid Quality: No
Product Size: 10 x 50 µL
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: No
T1 Phage - Resistant (tonA): No
Transformation Efficiency: > 1x10^8

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