MultiShot™ FlexPlate DH10B T1R Competent Cells
MultiShot&trade; FlexPlate DH10B T1<sup>R</sup> Competent Cells
Invitrogen™

MultiShot™ FlexPlate DH10B T1R Competent Cells

MultiShot FlexPlate DH10B T1R chemically competent E. coli cells are cloning competent cells pre-aliquoted in a 96-well PCR plate toRead more
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Catalog number C6481201
Price (USD)/ 1 x 96 well plate (20 µl/well)
598.00
-
Add to cart
Quantity:
1 x 96-well Plate
Price (USD)/ 1 x 96 well plate (20 µl/well)
598.00
Add to cart
MultiShot™ FlexPlate DH10B T1R Competent Cells
Catalog numberC6481201
Price (USD)/ 1 x 96 well plate (20 µl/well)
598.00
-
Add to cart

MultiShot FlexPlate DH10B T1R chemically competent E. coli cells are cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be conveniently divided into 8-well segments for flexibility in transformation throughput.

All of our T1R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Diverse DH10B T1R cloning capabilities
MultiShot FlexPlate DH10B T1R competent cells may be used in a variety of cloning applications as they are resistant to the effects of ligase and ligation buffers and provide transformation efficiencies of >1 x 108 cfu/μg with control plasmid DNA. The cells are ideal for cloning large plasmids and BACs and are suitable for cloning DNA that contains methylcytosine and methyladenine (i.e., genomic DNA).

MultiShot FlexPlate DH10B T1R Competent Cell features
mcrA, mcrBC, mrr, and hsdRMS elimination of restriction systems to allow construction of more representative genomic constructs
tonA (fhuA) genotype to confer resistance to T1 and T5 phage
endA1 for increased plasmid yield and quantity
recA1 for reduced occurrence of non-specific recombination in cloned DNA
lacZΔM15 for blue/white color screening of recombinant clones

Increase transformation productivity with MultiShot FlexWell format
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler.

Key benefits of the MultiShot FlexPlate format include:
• Increase throughput—compatible with automated liquid handling platforms
• Flexible 96-well plate may be divided into 8-wellsstrips
• Competent cells come pre-aliquoted in 96-well plates and ready for transformation

Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 lacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK rpsL nupG λ- tonA

Find the strain and format that you need
We offer many E. coli strains and formats of chemically competent and electrocompetent cells to meet your specific transformation needs. Choose from our MultiShot formatted comp cells for high-throughput options or email us for custom formatting information.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeChemically Competent Cells
Contains F' EpisomeNo
Cloning Methylated DNAYes
Antibiotic Resistance BacterialNo
Blue/White ScreeningYes (lacZΔM15)
High-throughput CompatibilityHigh
PlasmidMay be used for plasmids >100 kb
Preparing Unmethylated DNANo
Reduces RecombinationYes (recA1)
Shipping ConditionDry Ice
T1 Phage - Resistant (tonA)Yes
SpeciesE. coli (K12)
Format96-well Plate
Product LineMultishot™
Quantity1 x 96-well Plate
Unit Size1 x 96 well plate (20 µl/well)
Contents & Storage
• MultiShot FlexPlate DH10B T1R Competent Cells (1 x 96-well plate at 20 μL/well)
Store at –80°C.

• pUC19 DNA (50 μL at 10 pg/μL)
Store at –20°C.

• S.O.C. Medium (2 x 10 mL)
Store at 4°C or room temperature.