This review is about intracellular pH sensors, includingsmall fluorescent organic molecules, nanoparticles, andfluorescent proteins, e.g., GFP. It focuses on their preparations, photophysical properties, and advantages/disadvantagesfor intracellular pH measurements. The discussion is limitedto fluorescent indicators that have been applied to measureintracellular pH values since 1980. ... More
High-performance fiber-optic pH microsensors for practical physiological measurements using a dual-emission sensitive dye.
AuthorsSong A, Parus S, Kopelman R
JournalAnal Chem
PubMed ID9068274
A fast and durable ratiometric pH microoptode that is highly accurate, precise, sensitive, reversible, and reproducible over the physiological ranges of pH, ionic strength, and temperature has been developed. The sensing site consists of 5 (and 6)-carboxynaphthofluorescein (CNF) entrapped in a polyacrylamide gel matrix via photopolymerization at the silanized end ... More
Paradoxical effect of reagents for sulfhydryl and disulfide groups on human sperm capacitation and superoxide production.
Authorsde Lamirande E, Gagnon C
JournalFree Radic Biol Med
PubMed ID9823546
Spermatozoa must undergo capacitation prior to fertilization. In humans, this process appears regulated by oxidoreduction reactions. We investigated the possibility that these reactions involved the sulfhydryl-disulfide pair, which offers a reversible regulation of cellular processes. The effects of reagents targeted for sulfhydryl and disulfide groups on human sperm capacitation, superoxide ... More