Invitrogen™

One Shot™ BL21(DE3)pLysE Chemically Competent E. coli

Catalog number: C656503
Invitrogen™

One Shot™ BL21(DE3)pLysE Chemically Competent E. coli

Catalog number: C656503
Catalog Number
C656503
Unit Size
21 x 50 µL/tube
Price (USD)
Price: 366.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C65650321 x 50 µL/tube
Price: 366.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
BL21(DE3)pLysE E. coli are ideal for use with T7 promoter-based expression vectors. Like BL21(DE3)pLysS E. coli, BL21(DE3)pLysE carry the lambda DE3 lysogen. In addition, BL21(DE3)pLysE cells contain the pLysE plasmid, which constitutively expresses T7 lysozyme. T7 lysozyme reduces the basal expression of target genes by inhibiting T7 RNA polymerase. The pLysE plasmid in BL21(DE3)pLysE expresses higher levels of T7 lysozyme than the pLysS plasmid in BL21(DE3)pLysS.
The BL21(DE3)pLysE strain provides tighter control of T7 RNA polymerase, which is necessary when the recombinant protein to be expressed is toxic.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Competent Cell
Contains F' Episome
Lacks F' Episome
Efficiency
Subcloning Efficiency (10^6-10^7 cfu/µg)
Cell Type
Chemically Competent
Improves Plasmid Quality
No
Cloning Methylated DNA
No
Transformation Efficiency Level
Subcloning Efficiency (10^6-10^7 cfu⁄µg)
Bacterial or Yeast Strain
BL21(DE3)pLysE, BL21(DE3)
Blue/White Screening
No
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
No
T1 Phage - Resistant (tonA)
No
Species
E. coli
Promoter
T7
Format
One Shot
Product Line
One Shot™
Quantity
20 x 50 μL