Ribonuclease H renaturation gel assay using a fluorescent-labeled substrate.
AuthorsHan LY, Ma WP, Crouch RJ
JournalBiotechniques
PubMed ID9383560
Ribonucleases H (RNases H) are enzymes that specifically degrade the RNA of RNA-DNA hybrids. These enzymes are involved in DNA replication, reverse transcription (RT) and antisense oligodeoxyribonucleotide-mediated arrest of translation. One of the most valuable tools for assaying RNase H activity is the renaturation gel assay with which such activities ... More