CTS™ Soluble DynActivator™ CD3/CD28

Gibco™ CTS™ Soluble DynActivator™ CD3/CD28 is a soluble protein-based reagent designed to activate and expand enriched human T cell populations. The reagent is recommended to be used with Gibco™ CTS™ Detachable Dynabeads™ CD4 (Cat.no. A56994) and/ or Gibco™ CTS™ Detachable Dynabeads™ CD8 (Cat.no. A56995).

Features and benefits

• Activate and expand ≥1.5 billion T cells with a single vial.
  • High-capacity format supports larger-scale workflows while reducing reagent handling and vial changes.
• Simple, ready-to-use volumetric dosing.
  • Add directly to culture—no plate coating, or complex reagent handling.
• Soluble, protein-based format for easy removal.
  • Remove residual activation reagent through a simple media exchange.
• Greater flexibility in T cell processing workflows.
  • Decouple T cell isolation from activation, giving users greater freedom to optimize the timing, sequence, and configuration of individual process steps.
• More cells activated per vial. Lower cost per cell.
  • Activate and expand ≥1.5 x 10⁹ T cells from a single vial, enabling greater reagent efficiency and lower activation reagent cost per cell.

Additional product details

Gibco™ CTS™ Soluble DynActivator™ CD3/CD28 is coated with camelid-derived single-domain VHH antibodies that target CD3 and CD28 cell surface markers. The reagent can be sterile filtered, and excess (remaining) amount can be removed from cell culture by simple media replacement or by a washing step, e.g., centrifugation, using the CTS Rotea Counterflow Centrifugation System if needed. T cell expansion is achieved by culturing for up to 7-14 days. For longer cultivation, restimulation after 14 days may be necessary.

The recommended titers (product amounts per cell number and volume) have been found to efficiently stimulate CD3+ T cells; however, for special applications, it is recommended to further experimentally determine the optimal stimulation titer. Over-activation of T cells carries a risk of activation-induced cell death.

Gibco™ CTS™ Soluble DynActivator™ CD3/CD28 has been developed in combination with Thermo Fisher Scientific T cell culture media: CTS™ OpTmizer™ T Cell Expansion SFM (Cat.no. A1048501) or CTS™ OpTmizer™ One SFM (Cat.no. A5757201) supplemented with PeproGMP™ Human IL-2 Recombinant Protein (GMP200-02), or PeproGMP™ Human IL-7 Recombinant Protein (GMP200-07) and PeproGMP™ Human IL-15 Recombinant Protein (GMP200-15).