Muller¹ developed this medium in 1923. It was later modified by Kauffmann^2,3 with the addition of brilliant green and ox bile to suppress commensal organisms and thus improve the isolation of salmonellae.

The brilliant green dye used in the medium has been shown to be critical and Chroma or BDH brands should be used. It is essential that the dye is added as directed because heating the brilliant green or attempting to incorporate it in the basal medium seriously impairs its selective action.
The addition of novobiocin at 40mg per litre of broth was described by Jeffries⁴ to suppress the growth of Proteus species.

The addition of novobiocin at 40mg per litre of broth was described by Jeffries⁴ to suppress the growth of Proteus species.

Muller-Kauffmann Tetrathionate Broth should not be used if Salmonella typhi is suspected.

Muller-Kauffmann Tetrathionate Broth was used in a large-scale investigation between nine laboratories in eight different countries⁵.

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