Schandler Anaerobe Agar is free from thioglycollate and is used to create selective conditions under which delicate and more nutritionally exacting microorganisms of the intestinal tract develop, despite the presence of antagonistic organisms. Normally such fastidious microorganisms would be swamped by the growth of enterococci, coliform, bacilli, and other Gram-negative bacilli.

• Selective against non-target Gram-negative bacteria, especially Escherichia coli

Schaedler, Dubos, and Costello³ formulated this medium for the isolation of aerobic and anaerobic microorganisms from the gastro-intestinal tract of mice. Mata, Carrillo, and Villatoro⁴ modified the formula in their studies on anaerobic human fecal microflora.

Although thioglycollate is widely used in anaerobic media, to lower the redox potential in order to favor growth of anaerobic organisms, some workers have reported it to be inhibitory to some anaerobes.^2,3

Schaedler Anaerobe Agar contains cysteine hydrochloride and glucose, as reducing substances, with the advantage that cysteine inhibits the growth of Escherichia coli. Kari, Nagy, Kovacs & Hernadi⁴ have reported the inhibitory effect of cysteine on several enzymatic reactions of Escherichia coli in vitro.

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