Haemophilus species are nutritionally fastidious in nature and require complex media for growth. Because of the antagonism between some essential nutrients and certain antimicrobial agents, the complex media formulations make routine susceptibility testing of Haemophilus species difficult. Regular Mueller Hinton Agar requires the addition of blood to supply essential nutrients making medium opaque, rendering it unsuitable for antimicrobial susceptibility testing.

Haemophilus Test Medium is based on the formulation developed by Jorgensen et al.² which is now recommended by the United States CLSI. The transparency of the medium allows zones of inhibition to be read easily through the bottom of the Petri dish. Haemophilus Test Medium Base contains low levels of antimicrobial antagonists, which allows testing of trimethoprim/sulphamethoxazole to be carried out with greater confidence.

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