Thermo Scientific™

Modified Semi-solid Rappaport Vassiliadis Agar (ISO) (Dehydrated)

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Industrial Microbiology

Selectively enrich Salmonella spp. from food, animal feces, and environmental samples with Thermo Scientific™ Oxoid™ Modified Semi-solid Rappaport Vassiliadis (MRSV) Agar (ISO) (Dehydrated). The efficacy of the medium is based upon the ability of salmonellae to migrate through the selective medium ahead of competing Gram-negative, motile organisms and, thus, produce opaque halos of growth.
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Utilize motility enrichment on Modified Semi-solid Rappaport Vassiliadis Agar (ISO) for the isolation of Salmonella species from food and environmental samples associated with the primary stage of animal production (such as dust).
  • Simple and sensitive method for the isolation of salmonellae from food and environmental samples.
  • Incorporated into ISO 6579:2002+A1:2007 Annex D1, for the isolation of salmonellae from animal feces (such as poultry, pigs, and cattle).
  • Gram-positive flora is inhibited by novobiocin, low pH, high magnesium chloride concentration, and malachite green.
  • Isolation is based on the ability of salmonellae to migrate through the selective medium ahead of competing Gram-negative, motile organisms, thus producing opaque halos of growth.
  • Presumptive identification is achieved by subculture onto XLD Agar and a second Salmonella agar of choice.
Modified Semi-solid Rappaport Vassiliadis Agar (ISO) is based on the formulation described by De Smedt et al., which has been shown to detect more Salmonella positive samples than traditional enrichment procedures. 2

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1. ISO 6579:2002 +A1:2007 Annex D. 2. De Smedt J. M., Bolderdijk R., Rappold H. and Lautenschlaeger D. (1986) J. Food. Prot. 49. 510-514.