ChargeSwitch® gDNA Blood Kit, 50-100 µL This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: CS11000

Invitrogen™  Related applications: DNA Extraction

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Description

The ChargeSwitch® gDNA 50-100 µl Blood Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from small volumes of fresh or frozen, human blood in less than 15 minutes after lysate preparation.
• Optimized for higher-purity DNA extraction
• Compatible with high-throughput protocols
• Ideal for demanding downstream performance

Rapid, Three Step, pH Based Purification Protocol
The ChargeSwitch® gDNA 50-100 µl Blood Kit allows purification of genomic DNA from small volumes (up to 100 µl) of fresh or frozen, human blood treated with the anticoagulant EDTA or citrate typically in less than 15 minutes following sample preparation and lysis. ChargeSwitch® gDNA 50-100 µl Blood Kit uses magnetic beads conjugated to a unique ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Genomic DNA is isolated without the need for hazardous chemicals, centrifugation, or vacuum manifolds to yield up to 3 µg of gDNA from 50-100 µl of human blood (Table 1).

Note: gDNA may be isolated from heparin-treated blood; however, the DNA is not suitable for use in downstream applications such as PCR due to the presence of the heparin.

Compatible with Single Tube and High-Throughput Protocols
The ChargeSwitch® gDNA 50-100 µl Blood Kit is designed for automated processing of both small numbers of samples individually, as well as large numbers of samples using a liquid handling robot or a multi-channel pipette.

For processing up to 24 individual samples at a time, the MagnaRack™ two-piece magnetic separation tube rack is required. This rack consists of a magnetic base station and a removable tube rack, which can hold up to 24 microcentrifuge tubes and can be used with most ChargeSwitch® products.

For high-throughput applications utilizing 96 well blocks, the 96-Well Magnetic Separator is required. This rack can hold and separate up to 96 magnetic bead samples in a deep well plate when placed on top of the magnetic base station.

Improved Performance in Demanding Downstream Applications
The ChargeSwitch® gDNA 50-100 µl Blood Kit helps ensure reliable performance of the resulting gDNA in a variety of downstream applications, including PCR, restriction enzyme digestion, Southern blotting, and denaturing high-performance liquid chromatography (DHPLC).

For Research Use Only. Not intended for diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Blood
Isolation Technology: ChargeSwitch® Chemistry
Downstream Application: PCR, Southern Blotting, Cloning, Sequencing
High Throughput Compatibility: High Throughput-Compatible
Product Size: 50 preps
Green Features: Less hazardous, Less waste, Fewer resources used
Shipping Condition: Room Temperature

Contents & storage

All components of the ChargeSwitch® gDNA Blood Kits are shipped at room temperature. Upon receipt, store the Proteinase K at 4“C. Store all other components at room temperature. All components are guaranteed stable for 6 months if stored properly.

Components:

ChargeSwitch® Lysis Buffer (L12)
ChargeSwitch® Magnetic Beads
Proteinase K (20 mg/ml in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
ChargeSwitch® Purification Buffer (N5)
ChargeSwitch® Wash Buffer (W12)
ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

Documents

Manuals & protocols