Invitrogen™
ChargeSwitch™ gDNA Mini Tissue Kit
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Description
The ChargeSwitch® gDNA Mini Tissue Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from between 10-25 mg of tissue such as mouse tail, spleen, bone, and hair in less than 15 minutes after lysate preparation.• Optimized for higher-purity DNA extraction
• Improved performance with accompanying magnetic rack
• Cleaner purified plasmid for optimal downstream performance
High Quality, High Yield Plasmid DNA
The ChargeSwitch® gDNA Mini Tissue Kit allows purification of genomic DNA from 10-25 mg quantities of tissue typically in less than 15 minutes following sample preparation and lysis. ChargeSwitch® gDNA Mini Tissue Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Genomic DNA is isolated without the need for hazardous chemicals, centrifugation, or vacuum manifolds to yield up to 30 µg of gDNA from 0.5 cm mouse tail tips or approximately 25 mg of tissue. For spleen tissue, reduce tissue size to 10 mg.
Compatible with MagnaRack™ Magnetic Rack
The ChargeSwitch® gDNA Mini Tissue Kit requires the use of a magnetic rack. We recommend using the MagnaRack™ Magnetic Rack, which is a two-piece magnetic separation rack that consists of a magnetic base station and a removable tube rack. The tube rack fits onto the magnetic base station in two different positions associating the row of 12 neodymium magnets with a single row of 12 tubes for simple and easy "on the magnet" and "off the magnet" sample processing.
Improved Performance in Demanding Downstream Applications
The ChargeSwitch® gDNA Mini Tissue Kit ensures reliable performance of the resulting gDNA in a variety of downstream applications, including PCR, restriction enzyme digestion, and Southern blotting.
For Research Use Only. Not intended for diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Sample Type (General): | Tissue |
|---|---|
| Isolation Technology: | ChargeSwitch® Chemistry |
| Downstream Application: | Cloning, PCR, Sequencing, Southern Blotting |
| High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
| Product Line: | ChargeSwitch® |
| Product Size: | 25 preps |
| Shipping Condition: | Room Temperature |
Contents & storage
All components of the ChargeSwitch® gDNA Tissue Kits are shipped at room temperature. Upon receipt, store the Proteinase K and RNase A at 4“C. Store all other components at room temperature. All components are guaranteed stable for 6 months if stored properly.
Components:
ChargeSwitch® Lysis Buffer (L13)
ChargeSwitch® Lysis Buffer (L15)
ChargeSwitch® Magnetic Beads
Proteinase K (20 mg/ml in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
RNase A (5 mg/ml in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
ChargeSwitch® Purification Buffer (N5)
ChargeSwitch® Wash Buffer (W12)
ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Components:
ChargeSwitch® Lysis Buffer (L13)
ChargeSwitch® Lysis Buffer (L15)
ChargeSwitch® Magnetic Beads
Proteinase K (20 mg/ml in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
RNase A (5 mg/ml in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
ChargeSwitch® Purification Buffer (N5)
ChargeSwitch® Wash Buffer (W12)
ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
