ChargeSwitch® gDNA Mini Bacteria Kit This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: CS11301

Invitrogen™  Related applications: DNA Extraction

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Description

The ChargeSwitch® gDNA Mini Bacteria Kit uses magnetic bead-based technology to provide rapid and efficient purification of up to 12 µg of genomic DNA from up to 0.5 ml of Gram-positive or Gram-negative bacterial culture (Figure 1) or an individual colony in less than 15 minutes after lysate preparation.
• Optimized for higher-purity DNA extraction
• Improved performance with accompanying magnetic rack
• Cleaner purified plasmid for optimal downstream performance

High Quality, High Yield Plasmid DNA
The ChargeSwitch® gDNA Mini Bacteria Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Genomic DNA is isolated without the need for hazardous chemicals, centrifugation, or vacuum manifolds.

Compatible with MagnaRack™ Magnetic Rack
The ChargeSwitch® gDNA Mini Bacteria Kit requires the use of a magnetic rack. We recommend using the MagnaRack™ Magnetic Rack, which is a two-piece magnetic separation rack that consists of a magnetic base station and a removable tube rack. The tube rack fits onto the magnetic base station in two different positions associating the row of 12 neodymium magnets with a single row of 12 tubes for simple and easy "on the magnet" and "off the magnet" sample processing.

Improved Performance in Downstream Applications
The ChargeSwitch® gDNA Mini Tissue Kit ensures reliable performance of the resulting gDNA in a variety of downstream applications, including PCR, restriction enzyme digestion, cloning, Southern blotting, and sequencing.

For Research Use Only. Not intended for diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Bacteria
Isolation Technology: ChargeSwitch® Chemistry
Downstream Application: PCR, Cloning, Sequencing, Southern Blotting
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Product Size: 50 preps
Green Features: Less hazardous, Less waste, Fewer resources used
Shipping Condition: Room Temperature

Contents & storage

All components of the ChargeSwitch® gDNA (genomic DNA) Mini Bacteria Kit are shipped at room temperature. Upon receipt, store components as follows: Store Proteinase K at 4“C. Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4“C. Store the remaining kit components at room temperature. All components are guaranteed stable for 6 months when stored properly.

Components:

ChargeSwitch® Magnetic Beads (25 mg/ml in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
ChargeSwitch® Resuspension Buffer (R4)
ChargeSwitch® Lysis Buffer (L14)
ChargeSwitch® Binding Buffer (B8)
ChargeSwitch® Wash Buffer (W12)
ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Proteinase K (20 mg/ml in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
RNase A (5 mg/ml in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)

Documents

Manuals & protocols

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