ChargeSwitch®-Pro Plasmid Miniprep Kit

Catalog number: CS30050

Invitrogen™  Related applications: DNA Extraction

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Description

The ChargeSwitch®-Pro Plasmid Miniprep Kit generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. To accomplish this, the ChargeSwitch®-Pro Kit provides:

• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method

• Cleaner purified product for improved performance in downstream applications

• Ready-to-use, complete kit for a fast, hassle-free start

ChargeSwitch®-Pro Plasmid Miniprep Kit is ideal for purifying plasmid DNA from 1-5 ml overnight bacterial cultures, yielding up to 20 µg of plasmid DNA.

How it works

The ChargeSwitch® technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism. At low pH values (<6.5) the ChargeSwitch® surface ligand is positively charged and binds plasmid DNA. Raising the pH (>8.5) neutralizes the charge and elutes the bound nucleic acid. The ChargeSwitch® protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step (Figure 1, Table 1), making adapting to the ChargeSwitch® method effortless.

Better downstream results
DNA purified using the ChargeSwitch®-Pro Plasmid Miniprep Kit lacks potentially inhibitory compounds, improving DNA integrity for better downstream PCR performance (Figure 2). Expect to see improved cloning due to higher transformation efficiencies (Figure 3), faster restriction enzyme digestions (Figure 4), sequencing reads in excess 800 bp with Phred scores >20 (Figure 5), and a higher degree of supercoiling (Figure 6).

Contents and Storage:
The ChargeSwitch®-Pro Plasmid Miniprep Kit contains Miniprep Columns with Collection Tubes, Recovery Tubes, Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Sample Type (General): Bacteria
Downstream Application: PCR, Sequencing, Nucleic Acid Labeling, Next-Generation Sequencing, Transformation, In Vitro Transcription, Transfection, Cloning
Product Size: 50 preps
Final Product: Plasmid DNA
Isolation Technology: ChargeSwitch® Chemistry
Sample Type (Specific): Bacterial Culture
Shipping Condition: Room Temperature

Contents & storage

Store at room temperature.

Documents

Manuals & protocols

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