Invitrogen™

ChargeSwitch™-Pro Plasmid Miniprep Kit

Catalog number: CS30250
Invitrogen™

ChargeSwitch™-Pro Plasmid Miniprep Kit

Catalog number: CS30250
Catalog Number
CS30250
Unit Size
250 preps
Price (USD)
540.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
10 preps
Price (USD)
123.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
50 preps
Price (USD)
125.00
Your Price:
Availability
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
CS30250250 preps
540.00
Your Price:
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CS3001010 preps
123.00
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CS3005050 preps
125.00
Your Price:
***
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The ChargeSwitch™-Pro Plasmid Miniprep Kit generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. To accomplish this, the ChargeSwitch™-Pro Kit provides:

• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method

• Cleaner purified product for improved performance in downstream applications

• Ready-to-use, complete kit for a fast, hassle-free start

ChargeSwitch™-Pro Plasmid Miniprep Kit is ideal for purifying plasmid DNA from 1-5 ml overnight bacterial cultures, yielding up to 20 µg of plasmid DNA.

How it works

The ChargeSwitch™ technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism. At low pH values (<6.5) the ChargeSwitch™ surface ligand is positively charged and binds plasmid DNA. Raising the pH (>8.5) neutralizes the charge and elutes the bound nucleic acid. The ChargeSwitch™ protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step (Figure 1, Table 1), making adapting to the ChargeSwitch™ method effortless.

Better downstream results
DNA purified using the ChargeSwitch™-Pro Plasmid Miniprep Kit lacks potentially inhibitory compounds, improving DNA integrity for better downstream PCR performance (Figure 2). Expect to see improved cloning due to higher transformation efficiencies (Figure 3), faster restriction enzyme digestions (Figure 4), sequencing reads in excess 800 bp with Phred scores >20 (Figure 5), and a higher degree of supercoiling (Figure 6).

Contents and Storage:
The ChargeSwitch™-Pro Plasmid Miniprep Kit contains Miniprep Columns with Collection Tubes, Recovery Tubes, Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type
Spin Column
Format
Kit
Isolation Technology
ChargeSwitch™ Chemistry
Sample Type
Bacterial Culture
Final Product Type
Plasmid DNA
For Use With (Application)
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
250 Preps
Prep Scale
< 100 µg (Small-Scale) Plasmid DNA
Product Line
ChargeSwitch™
Product Type
Plasmid MiniPrep Kit
Quantity
250 Preps
Shipping Condition
Room Temperature
Target
Plasmid DNA

Contents & Storage

Store at room temperature.

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