Invitrogen™

ChargeSwitch™-Pro PCR Clean-Up Kit

Catalog number: CS32050
Invitrogen™

ChargeSwitch™-Pro PCR Clean-Up Kit

Catalog number: CS32050
Catalog Number
CS32050
Unit Size
50 preps
Price (USD)
Price: 237.00
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
250 preps
Price (USD)
Price: 718.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
CS3205050 preps
Price: 237.00
Your price:
-
CS32250250 preps
Price: 718.00
Your price:
-
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The ChargeSwitch™-Pro PCR Clean-up Kit purifies high-quality DNA fragments without the use of ethanol or harsh salts known to interfere with enzymatic reactions.

The ChargeSwitch™-Pro Kit provides:
• 100% ethanol-free, guanidinium-free, organic solvent-free protocols
• Improved downstream performance
• Complete kit for fast, hassle-free setup
• 20% fewer handling steps than most PCR clean-up protocols
• Purification of up to 500 µL of PCR product in one column


How It Works
ChargeSwitch™ technology employs a completely novel mechanism that replaces harsh reagents such as chaotropic salts, ethanol, or organic solvents and isopropanol precipitation with a simple ion-exchange procedure. In this method, DNA binds to a surface ligand that is positively charged at low pH, and is eluted when the pH is raised to 8.5 (Figure 1). The ChargeSwitch™-Pro protocol retains the ease and familiarity of common silica spin-column protocols with binding, washing, and elution steps (Figure 2).


Better Downstream Results
Since ChargeSwitch™ technology does not require the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification, the resulting DNA is free of enzymatic inhibitors and thus shows better downstream performance (Figure 3). These compounds are known to inhibit many enzymatic reactions such as PCR, cloning, restriction enzyme digestion, and array CGH labeling. Expect to see reduced variability in your sequencing applications, with reads in excess of 800 bp with Phred scores >20 (Figure 4); clean restriction enzyme digestions (Figure 5); and removal of dNTPs and primers in excess of 98% (Figure 6).

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Kit
Green Features
Fewer resources used and less waste, Less hazardous
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Product Line
ChargeSwitch™
Product Type
PCR Clean-Up Kit
Quantity
50 Preps
Shipping Condition
Room Temperature
Target
DNA from Enzymatic Reactions (e.g. PCR)
For Use With (Application)
Next-Generation Sequencing (NGS), Cloning, Standard PCR
Isolation Technology
ChargeSwitch™ Chemistry
No. of Reactions
50 Preps
Sample Type
PCR Products

Contents & Storage

The ChargeSwitch™-Pro PCR Clean-up Kit contains columns, collection tubes, recovery tubes, binding buffer, wash buffer, and elution buffer.

Store at room temperature. Guaranteed stable for 6 months when properly stored.

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