FGF-Basic Full Length CTS™ Recombinant Human Protein

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Description

Cell Therapy Systems CTS™ Recombinant Human FGF-basic (full length) is a bioactive protein intended for use in cell culture applications, including pre-clinical and clinical cell therapy studies. FGF-basic (FGFb), is involved in a number of biological processes including embryonic development and differentiation, and neuron differentiation, survival, and regeneration. FGFb is also involved in the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. The lyophilized, E. coli-expressed FGF-basic (full length) CTS™ Recombinant Human is verified for activity through dose-dependent proliferation of BALB/3T3 cells.

Qualified—helps reduce your burden in qualifying reagents for regulatory submission in cell therapy applications
Highly Pure—minimizes interference from other proteins or contaminants
Active—get more results with less protein

Simplified Transition into Clinical Research
GIBCO® Cell Therapy Systems (CTS™) products are of high quality and supplied with harmonized documentation such as Certificates of Analysis, Certificates of Origin, and access to authorization letters for our Drug Master Files, as appropriate. The GIBCO® CTS™ product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.

High Purity
To help verify that GIBCO® CTS™ Recombinant growth factors are of the highest quality, each protein is analyzed for purity, along with structural homogeneity, to help ensure a biologically active protein. The purity of each GIBCO® CTS™ recombinant growth factor is verified by SDS-PAGE to be over 95% pure and to have endotoxin levels below 0.1 ng/μg.

High Biological Activity
All GIBCO® CTS™ Recombinant growth factors are verified for activity. Each lot has its own ED50 determined using an activity assay based on a specific function of the growth factor. The activity of our FGF-basic (full length) CTS™ Recombinant Human growth factor is determined by the dose-dependent proliferation of BALB/3T3 cells to have an ED50 range of 0.1–1.0 ng/mL (specific activity: 1.0 × 107–1.0 × 106 units/mg). Optimal concentration for individual applications should be determined by a dose response assay.

Related Links
Learn More About GIBCO® Cell Therapy Systems (CTS™) Products
Cell Therapy Systems Technical Support Web Page
Our Full Collection of GIBCO® CTS™ Growth Factors
Our Full Collection of GIBCO® Growth Factors
For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals.

Specifications

Purity: 95 %
Species: Human
Activity: 0.1 ng/ml
Bioassay: Proliferation of BALB⁄3T3 cells
Expression: E. coli
Gene Aliases: FGF
Product Size: 1 mg
Protein Form: Full Length, Recombinant, Ligand
Purification: Sequential Chromatography
Protein Family: Growth Factors & Receptors
Endotoxin Level: < 0.1 ng/µg
Protein Subtype: FGFs (Fibroblast Growth Factors)
Molecular Weight: 17.2 kDa
Research Category: Neurobiology, Clinical Research, Differentiation, Stem Cell Research, Microbiology, Angiogenesis, Oncology
Validated Application: Cell Culture
Purity or Quality Grade: Carrier-Free
Protein Length or Portion (a.a.): 155 aa
Shipping Condition: Wet Ice

Contents & storage

2° to 8° C

Documents

Manuals & protocols

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