An 11-color flow cytometric assay for identifying, phenotyping, and assessing endocytic ability of peripheral blood dendritic cell subsets in a single platform.
AuthorsWang JC, Kobie JJ, Zhang L, Cochran M, Mosmann TR, Ritchlin CT, Quataert SA,
JournalJ Immunol Methods
PubMed ID19049809
Human peripheral blood dendritic cells (PBDC) are a rare population comprised of several distinctive subsets. Analysis of these cells has been hindered by their low frequency. In this study, we report a novel direct ex vivo 11-color flow cytometric assay that combines subset identification with analysis of activation status and ... More
Use of dextran beads for live analysis of the nuclear division and nuclear envelope breakdown/reformation cycles in the Drosophila embryo.
AuthorsKalpin RF, Daily DR, Sullivan W,
JournalBiotechniques
PubMed ID7530458
We employ fluorescently labeled dextran beads to follow cycles of nuclear division and nuclear envelope breakdown in living Drosophila embryos. When injected into syncytial Drosophila embryos, 3000 mol wt fluorescently labeled dextran beads concentrate in the interphase nucleus. Through confocal microscopy, undisrupted multiple cycles of nuclear division are readily followed. ... More
The steroidogenic acute regulatory protein homolog MLN64, a late endosomal cholesterol-binding protein.
AuthorsAlpy F, Stoeckel ME, Dierich A, Escola JM, Wendling C, Chenard MP, Vanier MT, Gruenberg J, Tomasetto C, Rio MC
JournalJ Biol Chem
PubMed ID11053434
MLN64 is a transmembrane protein that shares homology with the cholesterol binding domain (START domain) of the steroidogenic acute regulatory protein. The steroidogenic acute regulatory protein is located in the inner membrane of mitochondria, where it facilitates cholesterol import into the mitochondria. Crystallographic analysis showed that the START domain of ... More
Susceptibility to capillary plugging can predict brain region specific vessel loss with aging.
Authors
JournalJ Cereb Blood Flow Metab
PubMed ID31903837
α-Synuclein Dimers Impair Vesicle Fission during Clathrin-Mediated Synaptic Vesicle Recycling.
Authors
JournalFront Cell Neurosci
PubMed ID29321725
Ppargc1a Controls Ciliated Cell Development by Regulating Prostaglandin Biosynthesis.
Authors
JournalCell Rep
PubMed ID33176142
Organotypic primary blood vessel models of clear cell renal cell carcinoma for single-patient clinical trials.
Authors
JournalLab Chip
PubMed ID33103699
Lymph node fibroblastic reticular cells preserve a tolerogenic niche in allograft transplantation through laminin α4.
Authors
JournalJ Clin Invest
PubMed ID35775481
GW5074 Increases Microglial Phagocytic Activities: Potential Therapeutic Direction for Alzheimer's Disease.
Authors
JournalFront Cell Neurosci
PubMed ID35677758
Claudin-5 binder enhances focused ultrasound-mediated opening in an in vitro blood-brain barrier model.
Authors
JournalTheranostics
PubMed ID35265192
Bioinformatics Screening of Genes Specific for Well-Regenerating Vertebrates Reveals c-answer, a Regulator of Brain Development and Regeneration.
Authors
JournalCell Rep
PubMed ID31644900
Zebrafish mnx1 controls cell fate choice in the developing endocrine pancreas.
Authors
JournalDevelopment
PubMed ID21989909
Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS.