In vivo properties of an IgG3-IL-2 fusion protein. A general strategy for immune potentiation.
AuthorsHarvill ET, Fleming JM, Morrison SL
JournalJ Immunol
PubMed ID8816429
'In this report, we describe a strategy for enhancing the immunogenicity of a wide variety of Ags by linking them to IL-2 via an IgG3-IL-2 fusion protein with high affinity for a convenient hapten Ag, dansyl (DNS; N,N-dimethyl-1-aminonaphthalene-5-sulfonyl chloride). This fusion protein, anti-DNS-IgG3-IL-2, combines the functional characteristics of its constituents ... More
Ca2+-dependent interaction of 5-dimethylaminonaphthalene-1-sulfonyl-calmodulin with cyclic nucleotide phosphodiesterase, calcineurin, and troponin I.
AuthorsKincaid RL, Vaughan M, Osborne JC, Tkachuk VA
JournalJ Biol Chem
PubMed ID6286665
Fluorescent protein--dye conjugates. I. Heterogeneity of sites on serum albumin labeled by dansyl chloride.
AuthorsChen RF
JournalArch Biochem Biophys
PubMed ID5677176
Fluorescence resonance energy transfer between sites in G-actin. The spatial relationship between Cys-10, Tyr-69, Cys-374, the high-affinity metal and the nucleotide.
AuthorsBarden JA, dos Remedios CG
JournalEur J Biochem
PubMed ID3665911
'Intramonomer fluorescence resonance energy transfer spectroscopy was employed to investigate the spatial relationship between labels attached to the residues Cys-10, Tyr-69, Cys-374, the high-affinity metal binding site and the nucleotide binding site in G-actin. The separation between the fluorescence donor 5-(dimethylamino)naphthalene-1-sulphonyl (Dns) chloride (dansyl chloride) used to label Tyr-69 and ... More
Visualization of proteins by modification of lysines, cysteines, and phosphorylated serines facilitates sample preparation for microsequencing.
AuthorsHsi KL, O'Neill SA, Dupont DR, Yuan PM
JournalAnal Biochem
PubMed ID9527845
'A procedure for visualization and sensitive detection of protein during sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and subsequent sample preparation for sequence analysis is described. This procedure utilizes either fluorescent or visible tags for certain amino acids in protein molecules, e.g., lysines modified with dansyl/dabsyl chloride and cystines/cysteines or phosphorylated ... More
Mycothiol biosynthesis and metabolism. Cellular levels of potential intermediates in the biosynthesis and degradation of mycothiol in mycobacterium smegmatis.
AuthorsAnderberg SJ, Newton GL, Fahey RC
JournalJ Biol Chem
PubMed ID9804803
'Mycothiol (MSH; 1-D-myo-inosityl-2-(N-acetyl-L-cysteinyl)amido-2-deoxy-alpha-D- glucop yranoside (AcCys-GlcN-Ins)) is a novel thiol produced at millimolar levels by mycobacteria and other actinomycetes that do not make glutathione. We developed methods to determine the major components of MSH (AcCys, Cys-GlcN, AcCys-GlcN, Cys-GlcN-Ins, GlcN-Ins) in cell extracts. Mycobacterium smegmatis was shown to produce measurable levels ... More
Preparation of a fluorescent derivative of cytochrome b5 and its interaction with phospholipids.
AuthorsGilmore R, Glaser M
JournalBiochemistry
PubMed ID7082639
'A fluorescence derivative of bovine cytochrome b5 was prepared by using 5-(dimethylamino)naphthalene-1-sulfonyl chloride (dansyl chloride) in deoxycholate. Reaction conditions were established to specifically label the hydrophobic membrane-binding domain of the protein at a ratio of 0.9 +/- 0.1 dansyl group per cytochrome b5. Fluorescence measurements on the dansyl-labeled protein reflected ... More
Appraisal of four pre-column derivatization methods for the high-performance liquid chromatographic determination of free amino acids in biological materials.
AuthorsFürst P, Pollack L, Graser TA, Godel H, Stehle P
JournalJ Chromatogr
PubMed ID2324214
'Reversed-phase high-performance liquid chromatography (RP-HPLC) is a powerful method for assaying physiological amino acid concentrations in biological fluids. Four pre-column derivatization methods, with o-phthaldialdehyde (OPA), 9-fluorenylmethyl chloroformate (FMOC-Cl), phenyl isothiocyanate (PITC) and 1-dimethylaminonaphthalene-5-sulphonyl chloride (dansyl-Cl), were assessed with respect to their applicability in biological research. The methods permit the measurement ... More
Interaction of phalloidin with chemically modified actin.
AuthorsMiki M, Barden JA, dos Remedios CG, Phillips L, Hambly BD
JournalEur J Biochem
PubMed ID2952502
'Modification of Tyr-69 with tetranitromethane impairs the polymerizability of actin in accordance with the previous report [Lehrer, S. S. and Elzinga, M. (1972) Fed. Proc. 31, 502]. Phalloidin induces this chemically modified actin to form the same characteristic helical thread-like structure as normal F-actin. The filaments bind myosin heads and ... More
Rhodopsin in reconstituted phospholipid vesicles. 2. Rhodopsin-rhodopsin interactions detected by resonance energy transfer.
AuthorsBorochov-Neori H, Fortes PA, Montal M
JournalBiochemistry
PubMed ID6830760
'The interactions between rhodopsin molecules in a micellar detergent solution (octyl glucoside) and in reconstituted phospholipid vesicles were studied in the dark and after bleaching. Resonance energy transfer measurements were used to monitor the proximity between rhodopsin monomers conjugated with a fluorescent donor or a fluorescent acceptor. Reactive sulfhydryl groups ... More
Lysyl residues of cardiac myosin accessible to labeling with a fluorescent reagent, N-methyl-2-anilino-6-naphthalenesulfonyl chloride.
AuthorsHiratsuka T, Uchida K
JournalJ Biochem (Tokyo)
PubMed ID6450752
'Two mol of N-methyl-2-anilino-6-naphthalenesulfonyl (Mns) groups was preferentially incorporated into pig cardiac myosin in the absence of divalent metal ions, 1 mol rapidly and 1 mol slowly. In the presence of divalent metal ions. 1 mol was rapidly incorporated but subsequent incorporation was strongly suppressed. No substantial effect of incorporation ... More
Dimer-dimer interfaces of the lambda-repressor are different in liganded and free states.
AuthorsBandyopadhyay S, Mukhopadhyay C, Roy S
JournalBiochemistry
PubMed ID8664296
'Lambda-repressor dimers associate in solution to form tetramers and higher order structures. Dimer-dimer contact is also crucial in cooperative binding to adjacent operators. Fluorescence quenching studies indicate that the tryptophan 230 environment is significantly different in unliganded and adjacent operator-bound tetramers. Acrylodan attached to Cys 235, in a mutant F235C ... More
Evidence for assembly of small multidrug resistance proteins by a "two-faced" transmembrane helix.
AuthorsRath A, Melnyk RA, Deber CM
JournalJ Biol Chem
PubMed ID16574656
'Clinically significant bacterial resistance to drugs and cytotoxic compounds can be conferred by the energy-dependent efflux of toxicants, catalyzed by proteins embedded in the bacterial cell membrane. One such group of proteins, the small multidrug resistance family, are drug/proton antiporters that must oligomerize to function, a process that requires the ... More
Synthesis and pharmacological activity of fluorescent histamine H1 receptor antagonists related to mepyramine.
AuthorsLi L, Kracht J, Peng S, Bernhardt G, Buschauer A
JournalBioorg Med Chem Lett
PubMed ID12657255
'Fluorescently labeled histamine H(1) receptor antagonists were synthesized starting from N-demethylmepyramine by introduction of omega-aminoalkyl chains (2-8 methylene groups in length) followed by derivatization of the terminal NH(2) group with various fluorophores (fluorescein, naphthofluorescein, rhodamine, tetramethylrhodamine, BODIPY, dansyl, and nitrobenzoxadiazole (NBD)). On the isolated guinea pig ileum and in a ... More
Influences of metal ions on the reaction of amino and imino acids with fluorogenic reagents.
AuthorsMiyano H, Toyo'oka T, Imai K, Nakajima T
JournalAnal Biochem
PubMed ID3936376
'When a twofold excess of metal ions (Cu2+, Ni2+, Zn2+, Mg2+, Ca2+, Fe3+, and Al3+) was added to a reaction medium of amino acids (1-50 microM each) with the fluorogenic reagents NBD-F (4-fluoro-7-nitrobenzo-2-oxa-1,3-diazole), DNS-Cl (5-N,N-dimethylaminonaphthalenesulfonyl chloride), OPA (o-phthalaldehyde), and fluorescamine (4-phenylspiro[furan-2(3H),1''-phthalan]-3,3''-dione), a reduction of derivatization was observed. The effect was ... More
Inverse effects of dansylation of red blood cell membrane on band 3 protein-mediated transport of sulphate and chloride.
AuthorsLepke S, Passow H
JournalJ Physiol
PubMed ID6897945
'1. Dansylation of the red cell membrane produces inverse effects on SO(4) (2-) and Cl(-) equilibrium exchange. The former is enhanced by several orders of magnitude (Legrum, Fasold & Passow, 1980), the latter is inhibited. Both effects are potentiated after dansylation in the presence of 2-(4-amino-3-sulphophenyl)-6-methyl-7-benzothiazol sulphonic acid (APMB), a ... More
The scope of dansyl vs fluorescein label in fluorescence postlabeling assay for DNA damage.
AuthorsAzadnia A, Campbell R, Sharma M
JournalAnal Biochem
PubMed ID8074304
'The 5''-phosphate groups of the normal nucleotides of DNA (10 nmol) were labeled with two different fluorescent reagents, namely, dansyl chloride and fluorescein-isothiocyanate, via phosphoramidate derivatives of ethylene and hexamethylenediamines. Using a conventional detector, HPLC analysis showed a linear relationship of fluorescence signal as an integrated peak area and the ... More
Purification, amino terminal analysis, and peptide mapping of proteins after in situ postelectrophoretic fluorescent labeling.
AuthorsVera JC, Rivas CI, Cortés PA, Cárcamo JO, Delgado J
JournalAnal Biochem
PubMed ID3146233
'Proteins fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were stained in situ with either 5-(dimethylamino)-1-naphthalene sulfonyl chloride (dansyl chloride) or fluorescein isothiocyanate. This staining procedure can be carried out in less than 30 min without previous fixation of the proteins. It is not dependent on such factors as charge or ... More
Effects of specific chemical modification of actin.
AuthorsChantler PD, Gratzer WB
JournalEur J Biochem
PubMed ID128459
'G-actin has been nitrated with tetranitromethane in conditions that lead to the modification of one tyrosine residue. The reactive residue was found by earlier workers to be Tyr-69. The nitrated actin is conformationally similar to native G-actin, as judged by sedimentation velocity and circular dichroism analysis. A small proportion only ... More
Unfolding of trp repressor studied using fluorescence spectroscopic techniques.
AuthorsFernando T, Royer CA
JournalBiochemistry
PubMed ID1637807
'The unfolding properties of the trp repressor of Escherichia coli have been studied using a number of different time-resolved and steady-state fluorescence approaches. Denaturation by urea was monitored by the average fluorescence emission energy of the intrinsic tryptophan residues of the repressor. These data were consistent with a two-state transition ... More
Fluorogenic labeling of organophosphate pesticides with dansyl chloride. Application to residue analysis by high-pressure liquid chromatography and thin-layer chromatography.
AuthorsLawrence JE, Renault C, Frei RW
JournalJ Chromatogr
PubMed ID932151
'The analysis of some organophosphorus pesticides by fluorogenic labeling with dansyl chloride (5-dimethylaminonaphthalene--l-sulfonyl chloride) was investigated. The pesticides were hydrolysed in sodium hydroxide to the corresponding phenols. The reaction of dansyl chloride with the phenols was accomplished in a two-phase system. The resulting fluorescent derivatives were separated and analysed quantitatively ... More
Derivatization with fluorogenic benzofurazan reagents of amino acid enantiomers and their separation on a Pirkle type column.
AuthorsImai K, Fukushima T
JournalBiomed Chromatogr
PubMed ID8305859
'L- and D-Amino acids (Leu or Phe) were derivatized with fluorogenic reagents, 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), 4-(N,N-dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F), 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole (ABD-F) and 5-(N,N-dimethylamino)naphthalene-1-sulfonylchloride (DNS-CI), and separated on a Pirkle type column, Sumichiral OA 2500 (S) ((S)-1-naphthylglycyl-3,5-dinitrophenylamide silica gel) with a mobile phase of 20 mM ammonium acetate in methanol. The fluorometric detection of ... More
Subunit interactions in hemoglobin probed by fluorescence and high-pressure techniques.
AuthorsPin S, Royer CA, Gratton E, Alpert B, Weber G
JournalBiochemistry
PubMed ID2271588
'The dissociation of the subunits of human adult oxyhemoglobin has been investigated by using steady-state fluorescence anisotropy, multifrequency phase fluorometry, and high hydrostatic pressure. Human hemoglobin obtained by using two purification procedures (bulk preparation by centrifugation or further fractionation using anion-exchange chromatography) was labeled with an extrinsic fluorescent probe, 5-(dimethylamino)naphthalene-1-sulfonyl ... More
Synthesis and use of labelled nucleoside phosphoramidite building blocks bearing a reporter group: biotinyl, dinitrophenyl, pyrenyl and dansyl.
AuthorsRoget A, Bazin H, Teoule R
JournalNucleic Acids Res
PubMed ID2798121
'The synthesis of protected nucleoside phosphoramidites bearing various markers such as biotinyl, dinitrophenyl, dansyl and pyrenyl groups are reported. These labelled deoxynucleosides phosphoramidites were used for solid phase oligonucleotide synthesis in the same way than the usual protected phosphoramidities without any change in the synthetic cycle and the deprotection step. ... More
Molecular contacts between nebulin and actin: cross-linking of nebulin modules to the N-terminus of actin.
AuthorsShih CL, Chen MJ, Linse K, Wang K
JournalBiochemistry
PubMed ID9048566
'Nebulin, a giant actin binding protein, coextends with actin and is thought to form a composite thin filament in the skeletal muscle sarcomere. To understand the molecular interactions between nebulin and actin, we have applied chemical cross-linking techniques to define molecular contacts between actin and ND8, a two-module nebulin fragment ... More
Effect of cholesterol and charge on pore formation in bilayer vesicles by a pH-sensitive peptide.
AuthorsNicol F, Nir S, Szoka FC
JournalBiophys J
PubMed ID8968598
'The effect of cholesterol on the bilayer partitioning of the peptide GALA (WEAALAEALAEALAEHLAEALAEALEALAA) and its assembly into a pore in large unilamellar vesicles composed of neutral and negatively charged phospholipids has been determined. GALA undergoes a conformational change from a random coil to an amphipathic alpha-helix when the pH is ... More
Polarization fluorescence, spin label and ultracentrifugal studies of specific interaction of low molecular weight proteins with the Fc fragment of human immunoglobulin G.
AuthorsDudich EI, Dudich IV
JournalMol Immunol
PubMed ID6318093
'Low mol. wt (about 2000) proteins which were found in normal human serum formed complexes with the Fc fragment of IgG. The interaction constant was not less than 10(6) l/mole. These complexes dissociated on dilution of the protein solution to below 2 microM or decreasing the pH below 6. The ... More
A determination of the radial coordinate of Tyr-69 in F-actin using fluorescence energy transfer.
AuthorsMiki M, dos Remedios CG
JournalBiochem Int
PubMed ID2126434
'Fluorescence energy transfer was measured between Tyr-69 residues in an F-actin filament using 5-dimethylaminonaphthalene-1-sulfonyl chloride (DNS-Cl) as a fluorescence energy donor and 4-dimethylaminoazobenzene-4-sulfonyl chloride (DABSYL-Cl) as the acceptor. Both labels are covalently attached to Tyr-69 residues in an F-actin filament. Taking the helical structure of the F-actin filament into consideration, ... More
Determination of morphine and 6-acetylmorphine in plasma by high-performance liquid chromatography with fluorescence detection.
AuthorsBarrett DA, Shaw PN, Davis SS
JournalJ Chromatogr
PubMed ID1885707
'A method is described for the simultaneous determination of morphine and 6-acetylmorphine in small volumes of human plasma by normal-phase high-performance liquid chromatography using solid-phase extraction, dansyl derivatisation and fluorescence detection. The lower limits of quantitation in a 0.1-ml plasma sample are 10 ng/ml for morphine and 25 ng/ml for ... More
LC determination of aminoglutethimide enantiomers as dansyl and fluorescamine derivatives in tablet formulations.
AuthorsCesur N, Apak TI, Aboul-Enein HY, Ozkirimli S
JournalJ Pharm Biomed Anal
PubMed ID12008127
'Determination of dansyl (AG-DNS) and fluorescamine (AG-F) derivatives of rac-aminoglutethimide in tablet formulation by HPLC has been achieved on a cellulose tris-(3,5-dimethylphenyl carbamate), known as Chiralcel OD and OD-R under normal and reversed phase columns, respectively, using a fluorescence detector (lambda(ex), 360 nm; lambda(em), 530 nm for AG-DNS derivatives; lambda(ex), ... More
Dansyl labeled proteins: determination of extinction coefficienc and number of bound residues with radioactive dansyl chloride.
AuthorsChen RF
JournalAnal Biochem
PubMed ID5704757
Dansylation of amino acids for high-performance liquid chromatography analysis.
AuthorsTapuhi Y, Schmidt DE, Lindner W, Karger BL
JournalAnal Biochem
PubMed ID7304940
Rapid kinetic studies on calcium interactions with native and fluorescently labeled calmodulin.
AuthorsMalencik DA, Anderson SR, Shalitin Y, Schimerlik MI
JournalBiochem Biophys Res Commun
PubMed ID7306084
Reaction of reactive sulfhydryl groups of creatine kinase with dansyl chloride.
AuthorsBrown CS, Cunningham LW
JournalBiochemistry
PubMed ID5501646
Fluorescent labeling of proteins and a plasma membrane using cycloheptaamylose-dansyl chloride complex.
AuthorsKinoshita T, Iinuma F, Tsuji A
JournalAnal Biochem
PubMed ID4423747
Use of the dansyl reaction in biochemical analysis.
AuthorsSeiler N
JournalMethods Biochem Anal
PubMed ID4913442
The use of a cholinergic fluorescent probe for the study of the receptor proteolipid.
AuthorsWeber G, Borris DP, De Robertis E, Barrantes FJ, La Torre JL, Llorente de Carlin MC
JournalMol Pharmacol
PubMed ID5139564
The reaction of 1-dimethylaminonaphthalene-5-sulfonyl chloride (DANSC1) with erythrocyte membranes. A new look at "vectorial" membrane probes.
Fluorescence polarization analysis of various immunoglobulins. Dependence of rotational relaxation time on protein concentration and on ability to precipitate with antigen.
AuthorsDudich EI, Nezlin RS, Franek F
JournalFEBS Lett
PubMed ID566218
Fluorescent labelling of proteins on thin layers of solid dansyl chloride.
AuthorsSikorski AF, Daczynska RB
JournalBiochim Biophys Acta
PubMed ID7126580
Comparative studies of tyrosine modification in pancreatic phospholipases. 2. Properties of the nitrotyrosyl, aminotyrosyl, and dansylaminotyrosyl derivatives of pig, horse, and ox phospholipases A2 and their zymogens.
AuthorsMeyer H, Puijk WC, Dijkman R, Foda-van der Hoorn MM, Pattus F, Slotboom AJ, de Haas GH
JournalBiochemistry
PubMed ID38834
Aorta elasticae and tendon collagen reactivity to 8-anilino-1-naphthalene sulphate (ANS) and dansylchloride.
AuthorsVidal BC
JournalCell Mol Biol Incl Cyto Enzymol
PubMed ID7011559
Structural investigation of reconstituted chloroplast ATPase with fluorescence measurements.
AuthorsBaird BA, Pick U, Hammes GG
JournalJ Biol Chem
PubMed ID155694
Design of active-site-directed fluorescent probes and their reactions with biopolymers.
AuthorsHimel CM, Mayer RT, Cook LL
JournalJ Polym Sci [A1]
PubMed ID5433743
The rotational diffusion of thyroglobulin. A study of thyroglobulin conjugated with pyrenebutyrate and with dimethyl-aminonaphthalenesulfonate.
AuthorsRawitch AB, Hudson E, Weber G
JournalJ Biol Chem
PubMed ID4187703
Fluorescent conjugates of albumins as potential new reagents for the determination of bilirubin and its reserve binding capacity in body fluids.
AuthorsNavon G, Panigel R
JournalClin Chim Acta
PubMed ID759050
Interaction of creatine kinase from monkey brain with substrate: analysis of kinetics and fluorescence polarization.
AuthorsGrossman SH
JournalJ Neurochem
PubMed ID6875561
Structure of actin observed by fluorescence resonance energy transfer spectroscopy.
AuthorsMiki M, O'Donoghue SI, Dos Remedios CG
JournalJ Muscle Res Cell Motil
PubMed ID1534564
Fluorescent protein-dye conjugates. II. Gamma globulin conjugated with various dyes.
AuthorsChen RF
JournalArch Biochem Biophys
PubMed ID4186001
Preparation of fluorescent, cross-linking, and biotinylated calmodulin derivatives and their use in studies of calmodulin-activated phosphodiesterase and protein phosphatase.
AuthorsKincaid RL, Billingsley ML, Vaughan M
JournalMethods Enzymol
PubMed ID2842624
Fluorescent localization of contractile proteins in tissue culture cells.
AuthorsWang K, Feramisco JR, Ash JF
JournalMethods Enzymol
PubMed ID6750319
Derivatization of posttranslationally modified amino acids.
AuthorsTeerlink T
JournalJ Chromatogr B Biomed Appl
PubMed ID7820276
After a brief overview of posttranslational modifications of protein amino acids, the use of various derivatizing reagents for amino acid analysis is discussed. Derivatization and chromatographic separation of hydroxyproline, methylhistidine, and phosphorylated amino acids are discussed in detail to illustrate some of the strategies that can be applied to the ... More
Evidence for acetylated amino-terminal residues in hemocyanins.
AuthorsZatta P, Salvato B
JournalBoll Soc Ital Biol Sper
PubMed ID7317186
The NH2 amino acid terminals of hemocyanin from Carcinus maenas determined by the cyanate and by the dansyl-chloride methods gave very low yields. This gave values for the molecular weight which were abnormally high compared to those determined by physical methods. Assuming that the NH2 terminals are acetylated, the number ... More
[Scope and limitations of the analytical use of dansyl chloride, I: The reaction of aromatic sulfonyl chlorides with aliphatic tertiary amines: the microanalytical aspects of the Hinsberg test (author's transl)]
AuthorsWiechmann M
JournalHoppe Seylers Z Physiol Chem
PubMed ID924381
Contrary to the present understanding of the Hinsberg test, aliphatic tertiary amines (N,N-dimethylethylamine, bufotenin, hordenine, tropan alkaloids) are shown generally to react with aromatic sulfonyl chlorides yielding the N-substituted sulfonamide derivatives of the monodealkylated amines as the result of oxidative dealkylation of the aliphatic tertiary amines. Possible errors resulting from ... More
Fluorimetric determination of oxidised and reduced glutathione in cells and tissues by high-performance liquid chromatography following derivatization with dansyl chloride.
AuthorsMartin J, White IN
JournalJ Chromatogr
PubMed ID1770099
A high-performance liquid chromatographic method utilising fluorimetric detection of oxidised and reduced glutathione, following derivatization with dansyl chloride is described. Dansyl derivatives are separated on an aminopropyl silica column with a methanol-sodium acetate gradient system giving detection limits (signal-to-noise ratio = 2) of 1 pmol. This is in the order ... More
Position-specific incorporation of dansylated non-natural amino acids into streptavidin by using a four-base codon.
AuthorsHohsaka T, Muranaka N, Komiyama C, Matsui K, Takaura S, Abe R, Murakami H, Sisido M
JournalFEBS Lett
PubMed ID14988018
Novel non-natural amino acids carrying a dansyl fluorescent group were designed, synthesized, and incorporated into various positions of streptavidin by using a CGGG four-base codon in an Escherichia coli in vitro translation system. 2,6-Dansyl-aminophenylalanine (2,6-dnsAF) was found to be incorporated into the protein more efficiently than 1,5-dansyl-lysine, 2,6-dansyl-lysine, and 1,5-dansyl-aminophenylalanine. ... More
Characterization of protein-hapten conjugates. 2. Electrospray mass spectrometry of bovine serum albumin-hapten conjugates.
AuthorsAdamczyk M, Gebler JC, Mattingly PG
JournalBioconjug Chem
PubMed ID8853461
Fifteen hapten-bovine serum albumin (BSA) conjugates were prepared from five commercially available activated haptens. Each hapten was coupled to BSA at three different ratios. The conjugates were characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and two mass spectrometry (MS) methods: matrix-assisted laser desorption ionization (MALDI) and liquid chromatography-electrospray ... More
Studies on the mechanism of action of the histone kinase dependent on adenosine 3',5'-monophosphate. Investigation of protein-protein interaction by electron spin-resonance spectroscopy and stopped-flow methods.
AuthorsGabibov AG, Kochetkov SN, Sashchenko LP, Smirnov IV, Timofeev VP, Severin ES
JournalEur J Biochem
PubMed ID6301831
The interaction of the cAMP-dependent protein kinase catalytic subunit with its protein substrate, histone H1, was studied. The H1 molecule was specifically converted into the aminotyrosine-72 derivative. Fluorescent and spin labels were introduced into this residue. The changes in the ESR and fluorescence spectra of respective derivatives were observed upon ... More
Influence of fluorophore and linker composition on the pharmacology of fluorescent adenosine A1 receptor ligands.
AuthorsBaker JG, Middleton R, Adams L, May LT, Briddon SJ, Kellam B, Hill SJ,
JournalBr J Pharmacol
PubMed ID20105183
BACKGROUND AND PURPOSE: The introduction of fluorescence-based techniques, and in particular the development of fluorescent ligands, has allowed the study of G protein-coupled receptor pharmacology at the single cell and single molecule level. This study evaluated how the physicochemical nature of the linker and the fluorophore affected the pharmacological properties ... More
Preparation of fluorescent nonpeptidic neuropeptide Y receptor ligands: analogues of the quinazoline-type anti-obesity Y5 antagonist CGP 71683A.
AuthorsLi L, Mayer M, Schneider E, Schreiber E, Bernhardt G, Peng S, Buschauer A
JournalArch Pharm (Weinheim)
PubMed ID14677152
As part of a programme to develop fluorescence-based methods for the study of the interactions between G-protein coupled receptors (GPCRs) and their ligands the preparation of low molecular weight fluorescence-labelled neuropeptide Y (NPY) Y(5) antagonists is reported. The naphthylsulfonyl group in the potent quinazoline-type NPY Y(5) receptor antagonist CGP 71683A ... More
Fluorescence postlabeling assay of RNA modification.
AuthorsJain R, Isac TV, Sharma M
JournalBiochem Biophys Res Commun
PubMed ID7514398
A new approach has been developed to assay RNA modification by combining enzymatic digestion of RNA to nucleoside monophosphate and fluorescence postlabeling. Nuclease P1 digestion of modified RNA affords both normal and modified nucleotides. The nucleotides are labeled in-situ with dansyl chloride via phosphoramidate derivatives with ethylenediamine following a procedure ... More
Fluorogenic reactions for biomedical chromatography.
AuthorsOhkura Y, Kai M, Nohta H
JournalJ Chromatogr B Biomed Appl
PubMed ID7820282
A number of fluorogenic reactions, which have been used for HPLC detection systems by means of pre- and/or postcolumn derivatization, are surveyed with respect to both sensitivity and selectivity for the determination of biomedically important substances. For the derivatization of the substances, two types of fluorogenic reactions, fluorescence-generating and fluorescence-tagging, ... More
Fluorescent labeling of nitrocellulose-bound proteins at the nanogram level without changes in immunoreactivity.
AuthorsVera JC, Rivas C
JournalAnal Biochem
PubMed ID3142302
Proteins blotted on nitrocellulose were stained with either 5-dimethylamino-1-naphthalene-sulfonylchloride (dansyl chloride) or fluorescein isothiocyanate. In both cases the staining procedure can be completed in less than 30 min. The sensitivity for detecting fluorescent-labeled proteins on nitrocellulose was 0.5 ng using a dot test. This was accomplished by transparentizing the nitrocellulose ... More
Dansylation of hydroxyl and carboxylic acid functional groups.
AuthorsBartzatt R
JournalJ Biochem Biophys Methods
PubMed ID11245890
Fluorescent labeling of primary and secondary amines using dansyl chloride has been widely used in the past. Its application provides an extremely sensitive means to detect amine functional groups to amounts of less than 1 microg of material. This work describes a method for the dansylation of hydroxyl (-OH) and ... More
Unbalanced ribosomal protein synthesis in a strain of Escherichia coli containing a cloned, truncated 16-S ribosomal RNA gene.
AuthorsBarnsley PG, Sells BH
JournalEur J Biochem
PubMed ID6188614
An Escherichia coli K12 strain, carrying the promotor and proximal portion of the 16-S rRNA gene from rrnB cloned in the high-copy-number plasmid psF2124, has been examined for abnormalities in ribosome biogenesis. Both ribosomal RNA accumulation and ribosome content are depressed in this strain as compared to the control strain ... More
Chemical modification of rat liver microsomal cytochrome P-450: study of enzymic properties and membrane topology.
AuthorsKunz BC, Vergères G, Winterhalter KH, Richter C
JournalBiochim Biophys Acta
PubMed ID2012820
Isolated rat liver cytochrome P-450IIB1 was alkylated and acetylated at primary amino groups, and the position of the modified amino acids in the protein was identified. Alkylation of up to nine amino groups did not disturb the interaction of reconstituted P-450 and NADPH-cytochrome-P-450 reductase in a way that hydroxylation of ... More
Separation of alkylaminonaphthylenesulfonyl peptides and amin acids by high-performance liquid chromatography. Methods for measuring melanotropin inhibiting factor breakdown.
AuthorsHui KS, Salschutz M, Davis BA, Lajtha A
JournalJ Chromatogr
PubMed ID6104674
N,N-Dimethyl diethyl, dipropyl, dibutyl, and N-monoisopropylaminoaphthylenesulfonyl derivatives of melanotropin inhibiting factor (MIF) and its metabolites were prepared, and their chromatographic behavior was investigated with thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC), using five solvent systems on polyamide layers and ten solvent systems on muBondapak C18 and muBondapak phenyl columns. ... More
Evaluation of six fluorescent protein stains for use in flow microfluorometry.
AuthorsFreeman DA, Crissman HA
JournalStain Technol
PubMed ID52920
Flow microfluorometric (FMF) analysis of stained cells has provided protein distribution histograms for large populations of cells. Spectral data and staining protocols were evaluated for six fluorescent protein dyes suggested for staining cells in liquid suspension. The requirements for dyes and/or staining protocol included minimal cell clumping and cell loss, ... More
De novo identification of highly active fluorescent kappa opioid ligands from a rhodamine labeled tetrapeptide positional scanning library.
AuthorsHoughten RA, Dooley CT, Appel JR
JournalBioorg Med Chem Lett
PubMed ID15050634
Highly active fluorescent compounds having kappa opioid activity were identified following the screening in a kappa-specific radioligand binding assay of a positional scanning tetrapeptide combinatorial library in which every tetrapeptide was fluorescently labeled. Lissamine rhodamine B sulfonyl chloride was coupled to the N terminal of a mixture-based tetrapeptide positional scanning ... More
Fluorescence depolarization studies on the flexibility of myosin rod.
AuthorsHarvey SC, Cheung HC
JournalBiochemistry
PubMed ID921927
The single photon counting method has been used to measure the decay of fluorescence polarization anisotropy of myosin rods labeled with extrinsic fluorophores. Rods labeled with 8-anilino-1-naphthalenesulfonate (ANS) or 5-dimethylaminoaphthalene-1-sulfonyl chloride (DNS-Cl) exhibit negative rotational correlation times; the anisotropy increases with time. Possible artifactual causes for the negative decay times ... More
Fluorescent labeling of bacteriorhodopsin: implications for helix connections.
AuthorsRenthal R, Cothran M, Dawson N, Harris GJ
JournalBiochim Biophys Acta
PubMed ID3101736
Purple membrane from Halobacterium halobium was reacted with dansyl (5-dimethylamino-1-naphthalenyl fluorescent labels that have specificity for different protein side chains of bacteriorhodopsin. Dansyl chloride was found to react primarily with Lys-41. Dansyl hydrazine was coupled, with water-soluble carbodiimide, to Glu-74 and/or Asp-85, which was the major modified site after papain-cleavage ... More
Evaluation of eight fluorochrome combinations for simultaneous DNA-protein flow analyses.
AuthorsStöhr M, Vogt-Schaden M, Knobloch M, Vogel R, Futterman G
JournalStain Technol
PubMed ID83691
Eight fluorescent dye combinations for simultaneous DNA-protein staining have been evaluated spectroscopically and flow microfluorometrically: propidium iodide (PI) with fluoresceinisothiocyanate (FITC), fluorescamine (FC), and dansylchloride (DANS); diamidinophenylindole (DAPII) with sulphorhodamin (SR101), tetramethylrhodamin isothiocyanate (TRITC), and nitrobenzodiazole (NBD); acriflavine (AF) with stilbene isothiocyanate sulphonic acid (SITS), and DAPI. Three different experimental ... More
Determination of amphetamine-related compounds by high-performance liquid chromatography with chemiluminescence and fluorescence detections.
AuthorsHayakawa K, Hasegawa K, Imaizumi N, Wong OS, Miyazaki M
JournalJ Chromatogr
PubMed ID2722984
High-performance liquid chromatography with chemiluminescence detection has been established for the determination of trace levels of amphetamine-related compounds (APs) after fluorigenic derivatization. Bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide in acetonitrile was used as a post-column chemilumigenic reagent. As derivatization reagents, dansyl chloride (Dns-Cl), 4-fluoro-7-nitrobenzoxadiazole (NBD-F) and naphtalene-2,3-dicarbaldehyde (NDA) were compared. Dns-Cl ... More
Interaction of the N-terminus of chicken skeletal essential light chain 1 with F-actin.
Skeletal myosin has two isoforms of the essential light chain (ELC), called LC1 and LC3, which differ only in their N-terminal amino acid sequence. The LC1 has 41 additional residues containing seven pairs of Ala-Pro, which form an elongated structure, and two pairs of lysines located near the N-terminus. When ... More
1-N-glycyl beta-oligosaccharide derivatives as stable intermediates for the formation of glycoconjugate probes.
AuthorsManger ID, Rademacher TW, Dwek RA
JournalBiochemistry
PubMed ID1420188
Incubation of reducing sugars in ammonium bicarbonate was found to be a simple procedure for the formation of beta-D-glycosylamines of purified complex oligosaccharides in 70-80% yield. These provide valuable intermediates for the synthesis of a wide range of oligosaccharide probes and derivatives by acylation of the 1-amino function. The 1-amino ... More
Overexpression of epidermal growth factor receptor in NIH-3T3-transfected cells slows its lateral diffusion and rate of endocytosis.
AuthorsBenveniste M, Livneh E, Schlessinger J, Kam Z
JournalJ Cell Biol
PubMed ID3133376
Interactions between membrane proteins are believed to be important for the induction of transmembrane signaling. Endocytosis is one of the responses which is regulated by both intracellular and extracellular signals. To study such interactions, we have measured the lateral mobility and rate of endocytosis of epidermal growth factor receptor in ... More
Fluorescence resonance energy transfer measurements of distances in actin and myosin. A critical evaluation.
Authorsdos Remedios CG, Miki M, Barden JA
JournalJ Muscle Res Cell Motil
PubMed ID3298315
The contractile proteins actin and myosin are of considerable biological interest. They are essential for muscle contraction and in eukaryotic cells they play a crucial role in most contractile phenomena. Over the years since the first fluorescence resonance energy transfer (FRET) paper appeared, an extensive body of literature has accumulated ... More
Engineering modular protein interaction switches by sequence overlap.
AuthorsSallee NA, Yeh BJ, Lim WA
JournalJ Am Chem Soc
PubMed ID17381089
Many cellular signaling pathways contain proteins whose interactions change in response to upstream inputs, allowing for conditional activation or repression of the interaction based on the presence of the input molecule. The ability to engineer similar regulation into protein interaction elements would provide us with powerful tools for controlling cell ... More
Electrostatic interactions regulate desensitization of the nicotinic acetylcholine receptor.
AuthorsSong XZ, Pedersen SE
JournalBiophys J
PubMed ID10692319
To determine the importance of electrostatic interactions for agonist binding to the nicotinic acetylcholine receptor (AChR), we examined the affinity of the fluorescent agonist dansyl-C6-choline for the AChR. Increasing ionic strength decreased the binding affinity in a noncompetitive manner and increased the Hill coefficient of binding. Small cations did not ... More
Synthesis and characterization of the dansyltyrosine derivatives of porcine pancreatic colipase.
AuthorsMcIntyre JC, Schroeder F, Behnke WD
JournalBiochemistry
PubMed ID2328241
Steady-state and time-resolved fluorescence techniques were used to study dansyltyrosine derivatives of porcine pancreatic colipase. Nitration, reduction, acylation, and dansylation reactions were utilized to synthesize two fluorescently labeled colipases: (o-aminodansyltyrosine 55 porcine colipase) (DNStyr55PC) and o-aminodansyltyrosine 59 porcine colipase (DNStyr59PC). DNStyr55PC was 200% active, while the DNStyr59 derivative maintained 80% ... More
Influence of various fluorescent and non-fluorescent labels on the kinetics of the complex formation of alpha-chymotrypsin with basic pancreatic trypsin inhibitor (Kunitz).
AuthorsBösterling B, Engel J
JournalHoppe Seylers Z Physiol Chem
PubMed ID11169
The association of alpha-chymotrypsin with basic pancreatic trypsin inhibitor was studied using extrinsic signals produced by fluorescent and nonfluorescent labels. The reactive dyes were covalently bound to the proteins in the complexed state, in which the binding region was protected. The signals were sufficiently large to measure the complex formation ... More
Calmodulin supports both inactivation and facilitation of L-type calcium channels.
AuthorsZühlke RD, Pitt GS, Deisseroth K, Tsien RW, Reuter H
JournalNature
PubMed ID10335846
L-type Ca2+ channels support Ca2+ entry into cells, which triggers cardiac contraction, controls hormone secretion from endocrine cells and initiates transcriptional events that support learning and memory. These channels are examples of molecular signal-transduction units that regulate themselves through their own activity. Among the many types of voltage-gated Ca2+ channel, ... More
Tight binding of bulky fluorescent derivatives of adenosine to the low affinity E2ATP site leads to inhibition of Na+/K+-ATPase. Analysis of structural requirements of fluorescent ATP derivatives with a Koshland-Némethy-Filmer model of two interacting ATP sites.
AuthorsThoenges D, Amler E, Eckert T, Schoner W
JournalJ Biol Chem
PubMed ID9890953
A Koshland-Némethy-Filmer model of two cooperating ATP sites has previously been shown to explain the kinetics of inhibition of Na+/K+-ATPase (EC 3.6.1.37) by dansylated ATP (Thoenges, D., and Schoner, W. (1997) J. Biol. Chem. 272, 16315-16321). The present work demonstrates that this model adequately describes all types of interactions and ... More
Pathway of ATP hydrolysis by monomeric and dimeric kinesin.
AuthorsMoyer ML, Gilbert SP, Johnson KA
JournalBiochemistry
PubMed ID9454569
The ATPase mechanism for a monomeric Drosophila kinesin construct, K341, was determined by pre-steady-state kinetic methods and compared to dimeric kinesin, K401. We directly measured the kinetics of binding mantATP (a fluorescent ATP analog) to the microtubule K341 complex, the dissociation of K341 from the microtubule, and release of phosphate ... More
Domain motion in actin observed by fluorescence resonance energy transfer.
AuthorsMiki M, Kouyama T
JournalBiochemistry
PubMed ID8060983
Actin is composed of two well-separated globular domains which are further subdivided into two subdomains [Kabsch, W., Mannherz, H. G., Suck, D., Pai, E. F., & Holmes, K. C. (1990) Nature 347, 37-44]. Subdomains 1 and 2 constitute the small domain, and subdomains 3 and 4 comprise the large domain. ... More
A fluorescent calmodulin that reports the binding of hydrophobic inhibitory ligands.
AuthorsJohnson JD, Wittenauer LA
JournalBiochem J
PubMed ID6870843
Ca2+ binding to calmodulin in the pCa range 5.5-7.0 exposes hydrophobic sites that bind hydrophobic inhibitory ligands, including calmodulin antagonists, some Ca2+-antagonists and calmodulin-binding proteins. The binding of these hydrophobic ligands to calmodulin can be followed by the approx. 80% fluorescence increase they produce in dansylated (5-dimethylaminonaphthalene-1-sulphonylated) calmodulin (CDRDANS). In ... More
Extrinsic signals for monitoring the association reaction of proteins as introduced by fluorescent and non-fluorescent labels.
AuthorsBösterling B, Engel J, Steinemann A, Schramm HJ
JournalHoppe Seylers Z Physiol Chem
PubMed ID11168
Two known dansyl labels (I, II) and 5-[2-(iodoacetamido)ethylamino]-1-naphthalene-sulfonic acid (III) and three new azo-dyes (IV - VI) were covalently attached to alpha-chymotrypsin and to basic pancreatic trypsin inhibitor by four different reactive groups. In order to protect the contact region of the proteins the complex of the two proteins was ... More
Proximity between fluorescent probes attached to four essential lysyl residues in phosphoenolpyruvate carboxylase. A resonance energy transfer study.
Phosphoenolpyruvate carboxylase, purified from maize leaves, is rapidly inactivated by the fluorescence probe dansyl chloride. The loss of activity can be ascribed to the covalent modification of an R-NH2 group, presumably the epsilon-NH2 group of lysine. Analysis of the data by the statistical method of Tsou [Sci. Sin. 11, 1535-1558 ... More
Calmodulin-dependent enzymes undergo a protein-induced conformational change that is associated with their interactions with calmodulin.
AuthorsHuang S, Carlson GM, Cheung WY
JournalJ Biol Chem
PubMed ID8125988
The anionic hydrophobic (amphipathic) fluorescent probe 2-(p-toluidinyl)-naphthalene-6-sulfonate was used to investigate the surface hydrophobic properties of calmodulin (CaM)-dependent enzymes as follows: calcineurin, myosin light chain kinase, cyclic nucleotide phosphodiesterase, CaM-dependent protein kinase II, and the gamma-subunit of phosphorylase kinase. We found that certain domains of these enzymes that interacted with ... More
Enhancement of anion equilibrium exchange by dansylation of the red blood cell membrane.
AuthorsLegrum B, Fasold H, Passow H
JournalHoppe Seylers Z Physiol Chem
PubMed ID7450677
Dansylation of resealed red cell ghosts enhances the band 3 protein-mediated equilibrium exchange of sulfate ions. After dansylation, the specific anion transport inhibitor 4,4'-diisothiocyanato-dihydrostilbene 2,2'-disulfonate (H2DIDS) is still capable of combining with its original binding site on the band 3 protein and of producing the same high degree of inhibition ... More
The effect of apolipoprotein A-II on the structure and function of apolipoprotein A-I in a homogeneous reconstituted high density lipoprotein particle.
AuthorsDurbin DM, Jonas A
JournalJ Biol Chem
PubMed ID9395462
In this study we examined the effects of apoA-II on the structure and function of apoA-I in homogeneous reconstituted HDL (rHDL). First, we measured the binding of apoA-II to apoA-I-rHDL, containing dipalmitoylphosphatidylcholine or palmitoyloleoylphosphatidylcholine, and the degree of apoA-I displacement at various ratios of apolipoproteins. Using fluorescence methods, we determined ... More
Inhibition of calmodulin-activated smooth-muscle myosin light-chain kinase by calmodulin-binding peptides and fluorescent (phosphodiesterase-activating) calmodulin derivatives.
AuthorsTörök K, Cowley DJ, Brandmeier BD, Howell S, Aitken A, Trentham DR
JournalBiochemistry
PubMed ID9558358
Aspects of the biochemistry of calmodulin have been addressed that bear on its cell biological role as a mediator of Ca2+ regulation. Calmodulin-binding peptides derived from the amino acid sequence of smooth-muscle myosin light-chain kinase (MLCK) were characterized as inhibitors of calmodulin activation of MLCK-catalyzed phosphorylation of the smooth-muscle regulatory ... More
Development of a streak-camera-based time-resolved microscope fluorimeter and its application to studies of membrane fusion in single cells.
AuthorsKusumi A, Tsuji A, Murata M, Sako Y, Yoshizawa AC, Kagiwada S, Hayakawa T, Ohnishi S
JournalBiochemistry
PubMed ID2054350
A time-resolved microscope fluorimeter based on a synchroscan streak camera and a fast pulsed laser system has been developed to measure the fluorescence lifetime decay under the fluorescence microscope. This system allows one to measure the nanosecond fluorescence lifetimes of fluorophores in a small spot (0.8-6.3 microns diameter) in single ... More
Biologically active fluorescent derivatives of spinach calmodulin that report calmodulin target protein binding.
AuthorsMills JS, Walsh MP, Nemcek K, Johnson JD
JournalBiochemistry
PubMed ID3365375
Spinach calmodulin (CaM) has been labeled at cysteine-26 with the sulfhydryl-selective probe 2-(4-maleimidoanilino)naphthalene-6-sulfonic acid (MIANS) to produce MIANS-CaM. The interaction of MIANS-CaM with CaM binding proteins was studied by fluorescence enhancement accompanying the protein-protein interactions. MIANS-CaM bound to smooth muscle myosin light-chain kinase with a Kd of 9 nM, causing ... More
Detection of conformational changes in actin by fluorescence resonance energy transfer between tyrosine-69 and cysteine-374.
AuthorsMiki M
JournalBiochemistry
PubMed ID1932011
The distance between 5-(dimethylamino)naphthalene-1-sulfonyl chloride (dansyl chloride or DNS-Cl) attached to Tyr-69 and N-[[4-[4-(dimethylamino)phenyl]azo]phenyl]maleimide (DABMI) or N-[4-(dimethylamino)-3,5-dinitrophenyl]maleimide (DDPM) attached to Cys-374 in an actin monomer was measured to be 2.51 nm or 2.27 +/- 0.04 nm, respectively, by fluorescence resonance energy transfer. This distance does not change significantly when the ... More
Changes in the organization of the lipid bilayer of the plasma membrane during spermatogenesis and epididymal maturation.
Ram, bull, and mouse sperm cells were stained with several fluorescent membrane probes. In contrast to nonspecific probes, merocyanine 540 (MC540), which displays preferential binding to loosely packed phospholipids in model membranes, was specifically localized to the anterior portion of the head and the midpiece of mature sperm. To establish ... More
Purification, measurement, and tissue distribution of a dansyl-derivatized glycopeptide from low-molecular weight follicle-stimulating hormone-inhibitor-containing fractions of porcine follicular fluid.
AuthorsSluss PM, Branca AA, Ford JJ, Krishnan KA, Reichert LE
JournalBiol Reprod
PubMed ID2497807
We have used dansyl chloride (5-dimethylamino-1-naphthalenesulfonyl choloride) to form dansyl derivatives of amine-containing compounds in follicular fluid or highly purified fractions containing a low molecular weight (MW) inhibitor of follicle-stimulating hormone (FSH) binding to receptor (FSH-BI). This approach allowed sensitive detection of the derivatives based on their fluorescent properties. By ... More
Fluorescence studies of the secondary structure and orientation of a model ion channel peptide in phospholipid vesicles.
AuthorsChung LA, Lear JD, DeGrado WF
JournalBiochemistry
PubMed ID1378757
A 21-residue peptide of the sequence (LSSLLSL)3 forms ion channels when incorporated into planar lipid bilayer membranes of diphytanoylphosphatidylcholine (diPhy-PC). The frequency of channel openings increases with the applied voltage gradient. We investigated the molecular and structural mechanisms underlying this voltage dependence. A series of seven peptides, each containing a ... More
Fluorescence of equine platelet tropomyosin labeled with acrylodan.
AuthorsClark ID, Burtnick LD
JournalArch Biochem Biophys
PubMed ID3341759
Equine platelet tropomyosin was labeled with the sulfhydryl-specific fluorescent reagent 6-acryloyl-2-dimethylaminonaphthalene (acrylodan). The extent of labeling at 4 degrees C could be regulated between 0.5 and 1.3 acrylodans per tropomyosin chain by varying the reaction time from 1 to 4.5 h. Acrylodan-labeled platelet tropomyosin, AD-P-TM, was highly fluorescent, having an ... More
Models of the actin monomer and filament from fluorescence resonance-energy transfer.
AuthorsO'Donoghue SI, Hambly BD, dos Remedios CG
JournalEur J Biochem
PubMed ID1572360
We have developed algorithms for combining fluorescence resonance-energy transfer (FRET) efficiency measurements into structural models which predict the relative positions of the chemical groups used in FRET. We used these algorithms to construct models of the actin monomer and filament derived solely from FRET measurements based on seven distinct loci. ... More