The ratiometric mitochondrial membrane potential probe JC-9 exhibits green fluorescence that is independent of membrane potential and an increase in red fluorescence at hyperpolarized membrane potentials.
Given that JC-1 and JC-9 dyes are mitochondrial membrane potential indicators, they are designed to be used in live cells with active mitochondria. These dyes are not compatible with fixed cell staining.
For Research Use Only. Not for use in diagnostic procedures.